Detection of catalase activity with aldehyde-doped liquid crystals confined in microcapillaries
DC Field | Value | Language |
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dc.contributor.author | Rim, Jinseob | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-27T08:42:04Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-11-01 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3091 | - |
dc.description.abstract | In this study, a simple, rapid, and label-free sensor was developed for detecting the enzymatic activity of catalase (CAT) with liquid crystals (LCs) confined in microcapillaries. Inside a microcapillary functionalized with n-octyltrichlorosilane, aldehyde-doped LCs anchored radially so that a pattern of straight lines was observed under a polarized optical microscope (POM). However, once hydrogen peroxide (HP) oxidized the aldehyde into carboxylic acid, which has surface activity, the orientation of the LCs at the interface changed, resulting in a distinct pattern change, from straight to crossed. In this system, the enzymatic activity of CAT could be detected as it inhibits the oxidation by decomposing HP; as a result, the pattern changed back to the straight one. From the orientational and optical shift, the enzymatic activity of CAT was detected up to a concentration of 0.8 fM under mild experimental conditions and 8 aM at pH 9.0. This result suggests the need for further study of microcapillary systems to develop simple and sensitive sensors for biochemical interactions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.subject | HYDROGEN-PEROXIDE | - |
dc.subject | BIOSENSOR | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | SURFACES | - |
dc.subject | DEFENSE | - |
dc.subject | SYSTEM | - |
dc.subject | WATER | - |
dc.subject | ASSAY | - |
dc.title | Detection of catalase activity with aldehyde-doped liquid crystals confined in microcapillaries | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000447232800004 | - |
dc.identifier.doi | 10.1016/j.ab.2018.08.010 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.560, pp.19 - 23 | - |
dc.identifier.scopusid | 2-s2.0-85052971705 | - |
dc.citation.endPage | 23 | - |
dc.citation.startPage | 19 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 560 | - |
dc.contributor.affiliatedAuthor | Rim, Jinseob | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Liquid crystal | - |
dc.subject.keywordAuthor | 4-Cyano-4 &apos | - |
dc.subject.keywordAuthor | -pentylbiphenyl (5 C B) | - |
dc.subject.keywordAuthor | Microcapillary | - |
dc.subject.keywordAuthor | Hydrogen peroxide | - |
dc.subject.keywordAuthor | Catalase | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | DEFENSE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | ASSAY | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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