Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization
DC Field | Value | Language |
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dc.contributor.author | Baek, Mi-Ock | - |
dc.contributor.author | Song, Hae-In | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.contributor.author | Yoon, Mee-Sup | - |
dc.date.available | 2020-02-27T08:42:58Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-10-30 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3194 | - |
dc.description.abstract | Human endometrium decidualization, a differentiation process involving biochemical and morphological changes, is a prerequisite for embryo implantation and successful pregnancy. Here, we show that the mammalian target of rapamycin (mTOR) is a crucial regulator of 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP)-induced decidualization in human endometrial stromal cells. The level of mSin1 in mTOR complex 2 (mTORC2) and DEPTOR in mTOR complex 1 (mTORC1) decreases during 8-Br-cAMP-induced decidualization, resulting in decreased mTORC2 activity and increased mTORC1 activity. Notably, DEPTOR displacement increases the association between raptor and insulin receptor substrate-1 (IRS-1), facilitating IRS-1 phosphorylation at serine 636/639. Finally, both S473 and T308 phosphorylation of Akt are reduced during decidualization, followed by a decrease in forkhead box O1 (FOXO1) phosphorylation and an increase in the mRNA levels of the decidualization markers prolactin (PRL) and insulin-like growth factor-binding protein-1 (IGFBP-1). Taken together, our findings reveal a critical role for mTOR in decidualization, involving the differential regulation of mTORC1 and mTORC2. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.subject | ENDOMETRIAL STROMAL CELLS | - |
dc.subject | POSITIVE FEEDBACK LOOP | - |
dc.subject | MAMMALIAN TARGET | - |
dc.subject | PROGESTERONE-RECEPTOR | - |
dc.subject | PHOSPHATIDIC-ACID | - |
dc.subject | RAPAMYCIN MTOR | - |
dc.subject | CYCLIC-AMP | - |
dc.subject | STEM-CELLS | - |
dc.subject | CROSS-TALK | - |
dc.subject | IN-VITRO | - |
dc.title | Differential regulation of mTORC1 and mTORC2 is critical for 8-Br-cAMP-induced decidualization | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000448762500001 | - |
dc.identifier.doi | 10.1038/s12276-018-0165-3 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.50 | - |
dc.identifier.kciid | ART002397344 | - |
dc.identifier.scopusid | 2-s2.0-85055612201 | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 50 | - |
dc.contributor.affiliatedAuthor | Baek, Mi-Ock | - |
dc.contributor.affiliatedAuthor | Song, Hae-In | - |
dc.contributor.affiliatedAuthor | Yoon, Mee-Sup | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENDOMETRIAL STROMAL CELLS | - |
dc.subject.keywordPlus | POSITIVE FEEDBACK LOOP | - |
dc.subject.keywordPlus | MAMMALIAN TARGET | - |
dc.subject.keywordPlus | PROGESTERONE-RECEPTOR | - |
dc.subject.keywordPlus | PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | RAPAMYCIN MTOR | - |
dc.subject.keywordPlus | CYCLIC-AMP | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | CROSS-TALK | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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