aP2-Cre Mediated Ablation of GHS-R Attenuates Adiposity and Improves Insulin Sensitivity during Aging
DC Field | Value | Language |
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dc.contributor.author | Lin, Ligen | - |
dc.contributor.author | Lee, Jong Han | - |
dc.contributor.author | Wang, Ruitao | - |
dc.contributor.author | Wang, Ru | - |
dc.contributor.author | Sheikh-Hamad, David | - |
dc.contributor.author | Zang, Qun S. | - |
dc.contributor.author | Sun, Yuxiang | - |
dc.date.available | 2020-02-27T09:40:54Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3259 | - |
dc.description.abstract | Ghrelin via its receptor, the growth hormone secretagogue receptor (GHS-R), increases food intake and adiposity. The tissue-specific functions of GHS-R in peripheral tissues are mostly unknown. We previously reported that while GHS-R expression is very low in white and brown fat of young mice, expression increases during aging. To investigate whether GHS-R has cell-autonomous effects in adipose tissues, we generated aP2-Cre-mediated GHS-R knockdown mice (aP2-Cre/Ghsr(f/f)). We studied young (5-6 months) and old (15-17 months) aP2-Cre/Ghsr(f/f) mice and their age-matched controls. Interestingly, young aP2-Cre/Ghsr(f/f) mice had normal body weight but reduced fat; old mice showed pronounced reductions of both body weight and body fat. Calorimetry analysis revealed that aP2-Cre/Ghsr(f/f) mice had normal food intake and locomotor activity at both young and old age; but intriguingly, while energy expenditure was normal at young age, it was significantly increased at old age. Both young and old aP2-Cre/Ghsr(f/f) mice exhibited improved insulin sensitivity and glucose tolerance. Importantly, old aP2-Cre/Ghsr(f/f) mice maintained higher core body temperature at 4 degrees C, and showed higher expression of the thermogenic uncoupling protein 1 (UCP1) gene. The ex vivo studies further demonstrated that GHS-R deficient white adipocytes from old mice exhibit increased glucose uptake and lipolysis, promoting lipid mobilization. Despite the fact that the in vivo phenotypes of aP2-Cre/Ghsr(f/f) mice may not be exclusively determined by GHS-R knockdown in adipose tissues, our data support that GHS-R has cell-autonomous effects in adipocytes. The anabolic effect of GHS-R in adipocytes is more pronounced in aging, which likely contributes to age-associated obesity and insulin resistance. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.subject | HORMONE SECRETAGOGUE RECEPTOR | - |
dc.subject | DIET-INDUCED OBESITY | - |
dc.subject | GHRELIN RECEPTOR | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IN-VIVO | - |
dc.subject | KAPPA-B | - |
dc.subject | MICE | - |
dc.subject | TISSUE | - |
dc.subject | METABOLISM | - |
dc.subject | RESISTANCE | - |
dc.title | aP2-Cre Mediated Ablation of GHS-R Attenuates Adiposity and Improves Insulin Sensitivity during Aging | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000448951000157 | - |
dc.identifier.doi | 10.3390/ijms19103002 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.10 | - |
dc.identifier.scopusid | 2-s2.0-85054051541 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 19 | - |
dc.citation.number | 10 | - |
dc.contributor.affiliatedAuthor | Lee, Jong Han | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | ghrelin | - |
dc.subject.keywordAuthor | GHS-R | - |
dc.subject.keywordAuthor | tissue-specific knockdown mice | - |
dc.subject.keywordAuthor | thermogenesis | - |
dc.subject.keywordAuthor | UCP1 | - |
dc.subject.keywordAuthor | adipose tissues | - |
dc.subject.keywordPlus | HORMONE SECRETAGOGUE RECEPTOR | - |
dc.subject.keywordPlus | DIET-INDUCED OBESITY | - |
dc.subject.keywordPlus | GHRELIN RECEPTOR | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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