An electro-conductive plane heating element for rapid thermal lysis of bacterial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, YoungJoong | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.available | 2020-02-27T09:40:54Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3261 | - |
dc.description.abstract | In the process of developing a point-of-care testing device, we fabricated a plane heating element using carbon paste capable of thermal lysis. The plane heating element can generate heat of 150 degrees C within 60 s at 0.4 A power supply within 8 W. The developed plane heating element was very effective in the extraction of DNA from both Gram-negative and Gram-positive bacteria which was further confirmed by agarose gel electrophoresis. In this approach, we aimed to eliminate expensive, time-consuming and labor-intensive procedures for the lysis of bacterial cells to extract nucleic acids to detect pathogenic bacteria accurately and swiftly. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.subject | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject | POLYMERASE-CHAIN-REACTION | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | DNA | - |
dc.subject | IDENTIFICATION | - |
dc.subject | EXTRACTION | - |
dc.subject | PCR | - |
dc.title | An electro-conductive plane heating element for rapid thermal lysis of bacterial cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000449137600016 | - |
dc.identifier.doi | 10.1016/j.mimet.2018.09.012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGICAL METHODS, v.153, pp.99 - 103 | - |
dc.identifier.scopusid | 2-s2.0-85053751573 | - |
dc.citation.endPage | 103 | - |
dc.citation.startPage | 99 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.citation.volume | 153 | - |
dc.contributor.affiliatedAuthor | Kim, YoungJoong | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Carbon paste | - |
dc.subject.keywordAuthor | Plane heating element | - |
dc.subject.keywordAuthor | POCT | - |
dc.subject.keywordAuthor | Sample preparation | - |
dc.subject.keywordAuthor | Thermal lysis | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | EXTRACTION | - |
dc.subject.keywordPlus | PCR | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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