Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyeong Jin | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-27T09:41:36Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-09-01 | - |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3351 | - |
dc.description.abstract | DNA single-strand breaks (SSBs) have attracted much interest since they are highly related to carcinogenesis and ageing. Herein, we report a new liquid crystal (LC)-based sensor for the detection of DNA SSBs generated by reactive oxygen species (ROS) created from the Fenton reaction. The adsorption of single-stranded DNA (ssDNA) onto the cationic surfactant-laden aqueous/LC interface interferes with the surfactant layer, inducing a planar orientation of the LCs. However, the DNA SSBs generated by the Fenton reaction-produced ROS result in a decrease of the electrostatic interactions between the ssDNA and cationic surfactant molecules, causing rearrangement of the surfactant layer and reorientation of the LCs back to a homeotropic alignment. The changes in orientation of the LCs caused by the DNA SSBs are simply converted and observed as a shift from a bright optical image of the LCs to a dark one under a polarized light microscope. With this simple LC-based approach, the DNA SSBs could be detected more effectively and rapidly without any complex instrumentation or intricate processes. Therefore, our research provides a novel strategy for the detection of DNA damage as well as better insight into the DNA-damaging process. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.relation.isPartOf | ANALYTICAL BIOCHEMISTRY | - |
dc.subject | PROTEIN-BINDING | - |
dc.subject | FENTON REACTION | - |
dc.subject | DAMAGE | - |
dc.subject | INTERFACE | - |
dc.subject | REPAIR | - |
dc.subject | MECHANISM | - |
dc.subject | SURFACES | - |
dc.subject | CELLS | - |
dc.subject | CHAIN | - |
dc.title | Imaging DNA single-strand breaks generated by reactive oxygen species using a liquid crystal-based sensor | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000441368000001 | - |
dc.identifier.doi | 10.1016/j.ab.2018.06.009 | - |
dc.identifier.bibliographicCitation | ANALYTICAL BIOCHEMISTRY, v.556, pp.1 - 6 | - |
dc.identifier.scopusid | 2-s2.0-85048707293 | - |
dc.citation.endPage | 6 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | ANALYTICAL BIOCHEMISTRY | - |
dc.citation.volume | 556 | - |
dc.contributor.affiliatedAuthor | Kim, Hyeong Jin | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Liquid crystal (LC) | - |
dc.subject.keywordAuthor | 4-Cyano-4 &apos | - |
dc.subject.keywordAuthor | -pentylbiphenyl (5CB) | - |
dc.subject.keywordAuthor | DNA single-strand breaks (SSBs) | - |
dc.subject.keywordAuthor | Fenton reaction | - |
dc.subject.keywordAuthor | Reactive oxygen species (ROS) | - |
dc.subject.keywordAuthor | Orientational transition | - |
dc.subject.keywordPlus | PROTEIN-BINDING | - |
dc.subject.keywordPlus | FENTON REACTION | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | INTERFACE | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | CHAIN | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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