Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM)
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nhu Hoa Thi Tran | - |
dc.contributor.author | Kieu The Loan Trinh | - |
dc.contributor.author | Lee, Jun-Ho | - |
dc.contributor.author | Yoon, Won Jung | - |
dc.contributor.author | Ju, Heongkyu | - |
dc.date.available | 2020-02-27T09:41:58Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 2072-666X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3401 | - |
dc.description.abstract | We demonstrate the enhancement of fluorescence emission from a dye, 5-carboxyfluorescein (FAM), which couples with surface plasmons at the spectral channels of excitation and emission. Experiments and calculations revealed that bimetallic (gold-silver) plasmon, as compared to the monometallic ones, allowed such coupling to be enhanced, at both the spectral channels. We achieved the maximum fluorescence enhancement level of 46.5-fold, with markedly high reproducibility (coefficient of variation similar to 0.5%) at a FAM concentration of 10 nM. We also found that higher fluorescence enhancement was more likely to be reproducible. This encourages the use of this technology for practical applications in fluorescence-based biochemical assays. Moreover, we investigated a FAM concentration-dependent enhancement of fluorescence. It was found that fluorescence enhancement decreased and saturated at above 10 nM concentration possibly due to partial photo-bleaching of FAM molecules. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MICROMACHINES | - |
dc.subject | MOLECULAR FLUORESCENCE | - |
dc.subject | SPECTRAL PROPERTIES | - |
dc.subject | SILVER PARTICLES | - |
dc.subject | LABELED DNA | - |
dc.subject | METAL | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | OLIGONUCLEOTIDES | - |
dc.subject | EXCITATION | - |
dc.subject | SUBSTRATE | - |
dc.subject | RESONANCE | - |
dc.title | Fluorescence Enhancement Using Bimetal Surface Plasmon-Coupled Emission from 5-Carboxyfluorescein (FAM) | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000448399300042 | - |
dc.identifier.doi | 10.3390/mi9090460 | - |
dc.identifier.bibliographicCitation | MICROMACHINES, v.9, no.9 | - |
dc.identifier.scopusid | 2-s2.0-85066820664 | - |
dc.citation.title | MICROMACHINES | - |
dc.citation.volume | 9 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Nhu Hoa Thi Tran | - |
dc.contributor.affiliatedAuthor | Kieu The Loan Trinh | - |
dc.contributor.affiliatedAuthor | Yoon, Won Jung | - |
dc.contributor.affiliatedAuthor | Ju, Heongkyu | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | fluorescence enhancement | - |
dc.subject.keywordAuthor | surface plasmon resonance | - |
dc.subject.keywordAuthor | 5-carboxyfluorescein | - |
dc.subject.keywordAuthor | nano-biophotonics | - |
dc.subject.keywordAuthor | optical biosensors | - |
dc.subject.keywordPlus | MOLECULAR FLUORESCENCE | - |
dc.subject.keywordPlus | SPECTRAL PROPERTIES | - |
dc.subject.keywordPlus | SILVER PARTICLES | - |
dc.subject.keywordPlus | LABELED DNA | - |
dc.subject.keywordPlus | METAL | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | EXCITATION | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | RESONANCE | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.