Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis
DC Field | Value | Language |
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dc.contributor.author | Jang, Young-Rock | - |
dc.contributor.author | Song, Joon Seon | - |
dc.contributor.author | Jin, Choong Eun | - |
dc.contributor.author | Ryu, Byung-Han | - |
dc.contributor.author | Park, Se Yoon | - |
dc.contributor.author | Lee, Sang-Oh | - |
dc.contributor.author | Choi, Sang-Ho | - |
dc.contributor.author | Kim, Yang Soo | - |
dc.contributor.author | Woo, Jun Hee | - |
dc.contributor.author | Song, Jae-Kwan | - |
dc.contributor.author | Shin, Yong | - |
dc.contributor.author | Kim, Sung-Han | - |
dc.date.available | 2020-02-27T09:42:52Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 0025-7974 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3521 | - |
dc.description.abstract | Coxiella burnetii is a common cause of blood culture-negative infective endocarditis (IE). Molecular detection of C burnetii DNA in clinical specimens is a promising method of diagnosing Q fever endocarditis. Here, we examined the diagnostic utility of Q fever polymerase chain reaction (PCR) of formalin-fixed heart valve tissue from patients with blood culture-negative IE who underwent heart valve surgery. Clinical and laboratory data of patients with blood culture-negative IE who underwent heart valve surgery during a 6-year period and for whom biopsy tissues were available were reviewed retrospectively. Blood culture-positive IE patients who underwent heart valve surgery within the last 3 years were used as controls. Heart valve samples were cultured and also subjected to histological examination and PCR for Q fever, brucellosis, and bartonellosis. Data from 20 patients with blood culture-negative IE and 20 with blood culture-positive IE were analyzed. Eight cases of blood culture-negative IE were PCR-positive for C burnetii (40%; 95% confidence interval, 19-64). No specimen was PCR-positive for brucellosis or bartonellosis. Histologically, 4 of 8 specimens with a positive Q fever PCR result were characterized by clusters of multinucleated giant cells without a fibrin ring. None of 20 patients with blood culture-negative IE received anti-Coxiella antibiotic therapy due to lack of clinical suspicion. Six-month mortality was higher in the Q fever PCR-positive group than in the Q fever PCR-negative group [38% (3/8) vs 0% (0/12), P = .049). Of the 20 patients with blood culture-positive IE, none yielded a positive Q fever PCR result for valve tissue. Approximately 40% of patients with culture-negative IE who received heart valve surgery were PCR-positive for Q fever; patients without clinical suspicion suffered high mortality. These data suggest that Q fever IE in patients with culture-negative IE is often missed in routine clinical practice. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.relation.isPartOf | MEDICINE | - |
dc.subject | Q-FEVER ENDOCARDITIS | - |
dc.subject | BLOOD CULTURES | - |
dc.subject | CARDIAC VALVES | - |
dc.subject | DIAGNOSIS | - |
dc.subject | CRITERIA | - |
dc.title | Molecular detection of Coxiella burnetii in heart valve tissue from patients with culture-negative infective endocarditis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000446592300037 | - |
dc.identifier.doi | 10.1097/MD.0000000000011881 | - |
dc.identifier.bibliographicCitation | MEDICINE, v.97, no.34 | - |
dc.identifier.scopusid | 2-s2.0-85063713605 | - |
dc.citation.title | MEDICINE | - |
dc.citation.volume | 97 | - |
dc.citation.number | 34 | - |
dc.contributor.affiliatedAuthor | Jang, Young-Rock | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | diagnosis | - |
dc.subject.keywordAuthor | endocarditis | - |
dc.subject.keywordAuthor | polymerase chain reaction | - |
dc.subject.keywordAuthor | Q fever | - |
dc.subject.keywordPlus | Q-FEVER ENDOCARDITIS | - |
dc.subject.keywordPlus | BLOOD CULTURES | - |
dc.subject.keywordPlus | CARDIAC VALVES | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | CRITERIA | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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