Molecular mechanisms of immunomodulatory activity by polysaccharide isolated from the peels of Citrus unshiu
DC Field | Value | Language |
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dc.contributor.author | Shin, Myoung-Sook | - |
dc.contributor.author | Park, Su Beom | - |
dc.contributor.author | Shin, Kwang-Soon | - |
dc.date.available | 2020-02-27T10:41:44Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3673 | - |
dc.description.abstract | The extracts of the Citrus unshiu fruit induce numerous biological activities such as anticancer, anti-adipogenic, and antimicrobial effects. Furthermore, its peel has been used as a Chinese medicine for centuries and is consumed as a tea in China, Japan, and Korea. We investigated the effects of the polysaccharide isolated from C. unshiu peel (CPE-II) on cytoldne and inflammatory mediator production in RAW 264.7 macrophages and performed signal transduction experiments to identify the pathways involved in its actions. CPE-II exhibited macrophage-stimulatory activity by inducing the production of interleukin (IL)-6, tumor necrosis factor (TNF)-alpha and nitric oxide (NO) in RAW 264.7 macrophages. Signal transduction experiments showed that CPE-II phosphorylated mitogen-activated protein kinases (MAPKs) and nuclear factor (NF)-kappa B in RAW 264.7 cells in a concentration-dependent manner. The effects of CPE-II on IL-6/IL-12 and TNF-alpha production were completely suppressed by a specific inhibitor of c-Jun N-terminal kinase (JNK). Moreover, experiments with neutralizing antibodies showed that Toll-like receptor 2 (TLR2) and TLR4 were involved in the stimulation of IL-6 and NO production by CPE-II in RAW 264.7 cells. Collectively, these results suggest that the TLR2/4 and JNK pathways are essential for the CPE-II immune activity in RAW 264.7 cells. (C) 2018 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.subject | TOLL-LIKE RECEPTORS | - |
dc.subject | RHAMNOGALACTURONAN-II | - |
dc.subject | INNATE IMMUNITY | - |
dc.subject | MACROPHAGE | - |
dc.subject | LEAVES | - |
dc.subject | RECOGNITION | - |
dc.subject | EXPRESSION | - |
dc.subject | PATHWAYS | - |
dc.subject | FRACTION | - |
dc.subject | PECTINS | - |
dc.title | Molecular mechanisms of immunomodulatory activity by polysaccharide isolated from the peels of Citrus unshiu | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000430522400068 | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2018.02.006 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.112, pp.576 - 583 | - |
dc.identifier.scopusid | 2-s2.0-85041525593 | - |
dc.citation.endPage | 583 | - |
dc.citation.startPage | 576 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | - |
dc.citation.volume | 112 | - |
dc.contributor.affiliatedAuthor | Shin, Myoung-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Citrus unshiu peel | - |
dc.subject.keywordAuthor | Macrophages | - |
dc.subject.keywordAuthor | Polysaccharide | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTORS | - |
dc.subject.keywordPlus | RHAMNOGALACTURONAN-II | - |
dc.subject.keywordPlus | INNATE IMMUNITY | - |
dc.subject.keywordPlus | MACROPHAGE | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | FRACTION | - |
dc.subject.keywordPlus | PECTINS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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