Determination of isoorientin levels in rat plasma after oral administration of Vaccinum bracteatum Thunb. methanol extract by high-performance liquid chromatography-tandem mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | Kim, Min-Ji | - |
dc.contributor.author | Kwon, Seung-Hwan | - |
dc.contributor.author | Jang, Choon-Gon | - |
dc.contributor.author | Maeng, Han-Joo | - |
dc.date.available | 2020-02-27T10:41:55Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 0269-3879 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3694 | - |
dc.description.abstract | A simple, sensitive and rapid liquid chromatography tandem mass spectrometry method (LC-MS/MS) was developed and validated for the determination of plasma isoorientin levels in rats. After simple protein precipitation using methanol, chromatographic analysis was performed using a Synergi 4 polar-RP 80A column (150 x 2.0mm, 4m) under isocratic conditions and a mobile phase consisting of 0.1% formic acid in water and methanol (80:20, v/v) at a flow rate of 0.2mL/min. In positive electrospray ionization mode, the protonated precursor and product ion transitions of isoorientin (m/z 449.0 299.1) and of puerarin (the internal standard; m/z 417.1 297.1) were acquired by multiple reaction monitoring. Calibration curves obtained for plasma showed good linearity over the concentration range 1-1000ng/mL. The lower limit of quantification was 1ng/mL. Intra- and inter-day precisions were within 8.8% relative standard deviation. Accuracies ranged from 92.1 and 109.7%. The isoorientin stability in rat plasma under typical handling/storage conditions also found to be acceptable. The developed method was applied successfully to a pharmacokinetic study of isoorientin orally administered as the methanol extract of Vaccinium bracteatum Thunb. or administered as pure isoorientin. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | BIOMEDICAL CHROMATOGRAPHY | - |
dc.title | Determination of isoorientin levels in rat plasma after oral administration of Vaccinum bracteatum Thunb. methanol extract by high-performance liquid chromatography-tandem mass spectrometry | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000434061900005 | - |
dc.identifier.doi | 10.1002/bmc.4188 | - |
dc.identifier.bibliographicCitation | BIOMEDICAL CHROMATOGRAPHY, v.32, no.6 | - |
dc.identifier.scopusid | 2-s2.0-85041239695 | - |
dc.citation.title | BIOMEDICAL CHROMATOGRAPHY | - |
dc.citation.volume | 32 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Kim, Min-Ji | - |
dc.contributor.affiliatedAuthor | Maeng, Han-Joo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | isoorientin | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | pharmacokinetics | - |
dc.subject.keywordAuthor | plasma | - |
dc.subject.keywordAuthor | rat | - |
dc.subject.keywordAuthor | Vaccinium bracteatum Thunb | - |
dc.subject.keywordAuthor | methanol extract | - |
dc.subject.keywordPlus | INDUCED DIABETIC MICE | - |
dc.subject.keywordPlus | PUERARIA-TUBEROSA | - |
dc.subject.keywordPlus | LC-MS/MS | - |
dc.subject.keywordPlus | LEAVES | - |
dc.subject.keywordPlus | GLYCOSIDES | - |
dc.subject.keywordPlus | EXCRETION | - |
dc.subject.keywordPlus | URINE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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