Potential role of nucleoside diphosphate kinase in myricetin-induced selective apoptosis in colon cancer HCT-15 cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong Hyun | - |
dc.contributor.author | Choi, Yong Jun | - |
dc.contributor.author | Park, See-Hyoung | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.available | 2020-02-27T10:41:57Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3697 | - |
dc.description.abstract | The flavonoid myricetin (MYR) is derived from vegetables and fruits. It has been shown to exert anti-cancer effects in various cell lines; however, the exact mechanism underlying these effects is yet to be elucidated. In this study, we evaluated the anti-cancer effects induced by MYR treatment in colon cancer HCT-15 cells. To detect cell proliferation, we conducted MU assay and real time-cell electronic sensing (RT-CES). We next performed comet assay and Annexin V and PI staining to detect cellular apoptotic features. After that, we conducted two-dimensional electrophoresis (2-DE) analysis to identify apoptotic proteins. The results of this analysis revealed that eight spots were differentially expressed. Among the spots, we selected nucleoside diphosphate kinase (NDPK) for further investigation, as it has been shown to regulate cancer cell apoptosis and metastasis. After that, we conducted realtime-PCR and western blot to detect the expression of NDPK mRNA and protein and wound-healing assay to detect cell migration and invasion. Finally, we performed NDPK siRNA transfection study and the results showed that NDPK knockdown inhibited apoptosis. Based on these collective results, we suggest that MYR induces apoptosis in human colon cancer HCT-15 cells selectively by increasing the expression of NDPK and other caspase-regulated apoptosis proteins. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.subject | METASTASIS SUPPRESSOR NM23-H1 | - |
dc.subject | ADJUVANT THERAPY | - |
dc.subject | INHIBITION | - |
dc.subject | FLAVONOIDS | - |
dc.subject | INDUCTION | - |
dc.subject | EVOLUTION | - |
dc.subject | DEATH | - |
dc.title | Potential role of nucleoside diphosphate kinase in myricetin-induced selective apoptosis in colon cancer HCT-15 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000433656100035 | - |
dc.identifier.doi | 10.1016/j.fct.2018.04.053 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.116, pp.315 - 322 | - |
dc.identifier.scopusid | 2-s2.0-85046135393 | - |
dc.citation.endPage | 322 | - |
dc.citation.startPage | 315 | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 116 | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Hyun | - |
dc.contributor.affiliatedAuthor | Choi, Yong Jun | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Myricetin | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase-related proteins | - |
dc.subject.keywordAuthor | Nucleoside diphosphate kinase (NDPK) | - |
dc.subject.keywordPlus | METASTASIS SUPPRESSOR NM23-H1 | - |
dc.subject.keywordPlus | ADJUVANT THERAPY | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | DEATH | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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