Anti-Inflammatory Effects of Angelica sinensis (Oliv.) Diels Water Extract on RAW 264.7 Induced with Lipopolysaccharide
DC Field | Value | Language |
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dc.contributor.author | Kim, Young-Jin | - |
dc.contributor.author | Lee, Ji Young | - |
dc.contributor.author | Kim, Hyun-Ju | - |
dc.contributor.author | Kim, Do-Hoon | - |
dc.contributor.author | Lee, Tae Hee | - |
dc.contributor.author | Kang, Mi Suk | - |
dc.contributor.author | Park, Wansu | - |
dc.date.available | 2020-02-27T10:42:46Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3810 | - |
dc.description.abstract | The dry root of Angelica sinensis (Oliv.) Diels, also known as female ginseng, is a popular herbal drug amongst women, used to treat a variety of health issues and cardiovascular diseases. The aim of this study is to evaluate the detailed molecular mechanism for anti-inflammatory effects of Angelica sinensis root water extract (ASW). The anti-inflammatory effect of ASW on lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages was evaluated by the tetrazolium-based colorimetric assay (MTT), Griess reagent assay, multiplex cytokine assay, real time reverse transcription polymerase chain reaction (RT-PCR), and Fluo-4 calcium assay. ASW restored cell viability in RAW 264.7 at concentrations of up to 200 mu g/mL. ASW showed notable anti-inflammatory effects. ASW exhibited IC50 = 954.3, 387.3, 191.7, 317.8, 1267.0, 347.0, 110.1, 573.6, 1171.0, 732.6, 980.8, 125.0, and 257.0 mu g/mL for interleukin (IL)-6, tumor necrosis factor (TNF)-, monocyte chemotactic activating factor (MCP)-1, regulated on activation, normal T cell expressed and secreted (RANTES), granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), lipopolysaccharide-induced CXC chemokine (LIX), macrophage inflammatory protein (MIP)-1, MIP-1, MIP-2, IL-10, and intracellular calcium, respectively. Additionally, ASW inhibited the LPS-induced production of nitric oxide and the LPS-induced mRNA expression of CHOP (GADD153), Janus kinase 2 (JAK2), signal transducers and activators of transcription 1 (STAT1), first apoptosis signal receptor (FAS), and c-Fos, NOS2, and PTGS2 (COX2) in RAW 264.7 significantly (p < 0.05). Data suggest that ASW exerts an anti-inflammatory effect on LPS-induced RAW 264.7 via NO-bursting/calcium-mediated JAK-STAT pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | NUTRIENTS | - |
dc.title | Anti-Inflammatory Effects of Angelica sinensis (Oliv.) Diels Water Extract on RAW 264.7 Induced with Lipopolysaccharide | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000435196000121 | - |
dc.identifier.doi | 10.3390/nu10050647 | - |
dc.identifier.bibliographicCitation | NUTRIENTS, v.10, no.5 | - |
dc.identifier.scopusid | 2-s2.0-85047363135 | - |
dc.citation.title | NUTRIENTS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 5 | - |
dc.contributor.affiliatedAuthor | Kim, Young-Jin | - |
dc.contributor.affiliatedAuthor | Lee, Ji Young | - |
dc.contributor.affiliatedAuthor | Kim, Hyun-Ju | - |
dc.contributor.affiliatedAuthor | Kim, Do-Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Tae Hee | - |
dc.contributor.affiliatedAuthor | Kang, Mi Suk | - |
dc.contributor.affiliatedAuthor | Park, Wansu | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Angelica sinensis | - |
dc.subject.keywordAuthor | anti-inflammatory | - |
dc.subject.keywordAuthor | macrophage | - |
dc.subject.keywordAuthor | cytokine | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | nitric oxide | - |
dc.subject.keywordAuthor | JAK-STAT | - |
dc.subject.keywordPlus | POLYINOSINIC-POLYCYTIDYLIC ACID | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CHOP | - |
dc.relation.journalResearchArea | Nutrition & Dietetics | - |
dc.relation.journalWebOfScienceCategory | Nutrition & Dietetics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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