Liquiritigenin prevents palmitate-induced beta-cell apoptosis via estrogen receptor-mediated AKT activation
DC Field | Value | Language |
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dc.contributor.author | Bae, Gong Deuk | - |
dc.contributor.author | Park, Eun-Young | - |
dc.contributor.author | Baek, Dong Jae | - |
dc.contributor.author | Jun, Hee-Sook | - |
dc.contributor.author | Oh, Yoon Sin | - |
dc.date.available | 2020-02-27T11:40:43Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 0753-3322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3843 | - |
dc.description.abstract | Liquiritigenin (LQ) is a major active component of licorice root, which is a flavone used for treating many diseases, including diabetes. LQ has been shown to exhibit a glucose-lowering effect in diabetic mice. Therefore, we investigated the potential of LQ to protect against lipotoxicity-induced beta-cell apoptosis and the underlying molecular mechanisms. Exposure of INS-1 rat insulinoma cells to LQ significantly increased cell viability and blocked palmitate (PA)-induced apoptosis, as evidenced by the reduction of Annexin-V-stained cells, cleaved caspase-3 levels, and poly (ADP-ribose) polymerase (PARP) activity, as well as upregulation of Bcl-2 expression. Moreover, LQ treatment significantly reduced the endoplasmic reticulum (ER) stress response by reducing phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (PERK), phosphorylated eIF-2a, and CHOP expression in PA-treated INS-1 cells. The anti-apoptotic effect of LQ treatment was reversed through co-treatment with fulvestrant, a specific inhibitor of the estrogen receptor. LQ also increased AKT phosphorylation, and inactivation of this molecular event failed to decrease PERK phosphorylation with LQ treatment in PA-treated INS-1 cells. This effect was further accompanied by an inability to recover cell viability. These results suggest that LQ protects INS-1 cells from lipotoxicity-induced apoptosis by suppressing ER stress. We conclude that estrogen receptor-mediated AKT phosphorylation is one of the mechanisms contributing to the anti-apoptotic effect of LQ. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | - |
dc.relation.isPartOf | BIOMEDICINE & PHARMACOTHERAPY | - |
dc.title | Liquiritigenin prevents palmitate-induced beta-cell apoptosis via estrogen receptor-mediated AKT activation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000429586400041 | - |
dc.identifier.doi | 10.1016/j.biopha.2018.02.097 | - |
dc.identifier.bibliographicCitation | BIOMEDICINE & PHARMACOTHERAPY, v.101, pp.348 - 354 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85042879425 | - |
dc.citation.endPage | 354 | - |
dc.citation.startPage | 348 | - |
dc.citation.title | BIOMEDICINE & PHARMACOTHERAPY | - |
dc.citation.volume | 101 | - |
dc.contributor.affiliatedAuthor | Bae, Gong Deuk | - |
dc.contributor.affiliatedAuthor | Jun, Hee-Sook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Liquiritigenin | - |
dc.subject.keywordAuthor | Beta-cell | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Endoplasmic reticulum stress | - |
dc.subject.keywordAuthor | AKT | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM STRESS | - |
dc.subject.keywordPlus | ER STRESS | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | HYPERGLYCEMIA | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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