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CRISPR-Cas9-mediated generation of obese and diabetic mouse models

Authors
Roh, Jae-ilLee, JunghoonPark, Seong UkKang, Young-ShinLee, JaehoonOh, Ah-ReumChoi, Dong JoonCha, Ji-YoungLee, Han-Woong
Issue Date
Apr-2018
Publisher
INT PRESS EDITING CENTRE INC
Keywords
CRISPR-Cas9; dbldb; leptin; leptin receptor; oblob
Citation
EXPERIMENTAL ANIMALS, v.67, no.2, pp.229 - 237
Journal Title
EXPERIMENTAL ANIMALS
Volume
67
Number
2
Start Page
229
End Page
237
URI
https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3907
ISSN
1341-1357
Abstract
Mouse models of obesity (oblob) and diabetes (dbldb) in which the leptin (Lep) and leptin receptor (Lepr) genes have been mutated, respectively, have contributed to a better understanding of human obesity and type 2 diabetes and to the prevention, diagnosis, and treatment of these metabolic diseases. In this study, we report the first CRISPR-Cas9-induced Lep and Lepr knockout (KO) mouse models by co-microinjection of Cas9 mRNA and sgRNAs that specifically targeted Lep or Lepr in C57BL/6J embryos. Our newly established Lep and Lepr KO mouse models showed phenotypic disorders nearly identical to those found in oblob and dbldb mice, such as an increase in body weight, hyperglycemia, and hepatic steatosis. Thus, Cas9-generated Lep and Lepr KO mouse lines will be easier for genotyping, to maintain the lines, and to use for future obesity and diabetes research.
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