Optimization of Cytokine Milieu to Reproduce Atopic Dermatitis-related Gene Expression in HaCaT Keratinocyte Cell Line
DC Field | Value | Language |
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dc.contributor.author | Kim, Hee Joo | - |
dc.contributor.author | Baek, Jinok | - |
dc.contributor.author | Lee, Jong Rok | - |
dc.contributor.author | Roh, Joo Young | - |
dc.contributor.author | Jung, YunJae | - |
dc.date.available | 2020-02-27T11:41:18Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 1598-2629 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3915 | - |
dc.description.abstract | Although atopic dermatitis (AD) is characterized by cytokine production predominantly mediated by T helper (Th) 2 cells, AD pathogenesis also involves innate immune and Th1 cells. To optimize the cytokine milieu required for accurate reproduction of AD-related gene expression profile in vitro, we evaluated the expression pattern of CCL22, CCL17, IL5, IL13, IL33, IL25, TSLP, FLG, and LOR in human lesional AD skin and cytokine-stimulated HaCaT cells. An increase in Th2 mediators (IL5, IL13, CCL22, CCL17, IL25, IL33, and TSLP) and a decrease in genes related to cornified cell envelope (filaggrin and loricrin) were observed in human AD lesions. Innate (tumor necrosis factor-alpha) and/or Th1/Th2 adaptive cytokines (interferon-gamma/IL-4) were required for inducing these inflammatory changes in HaCaT cells, implying that a complex network of innate, Th1, and Th2 cytokines drives AD-like changes. Therefore, stimulation with various combinations of cytokines, beyond Th2 polarization, is necessary when HaCaT cell line is used to study genetic changes implicated in AD pathogenesis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREA ASSOC IMMUNOLOGISTS | - |
dc.relation.isPartOf | IMMUNE NETWORK | - |
dc.subject | EPITHELIUM-DERIVED CYTOKINES | - |
dc.subject | TNF-ALPHA | - |
dc.subject | HUMAN SKIN | - |
dc.subject | IL-33 | - |
dc.subject | INFLAMMATION | - |
dc.subject | MODULATION | - |
dc.subject | FILAGGRIN | - |
dc.subject | SYSTEM | - |
dc.subject | MODEL | - |
dc.subject | GAMMA | - |
dc.title | Optimization of Cytokine Milieu to Reproduce Atopic Dermatitis-related Gene Expression in HaCaT Keratinocyte Cell Line | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000434283900005 | - |
dc.identifier.doi | 10.4110/in.2018.18.e9 | - |
dc.identifier.bibliographicCitation | IMMUNE NETWORK, v.18, no.2 | - |
dc.identifier.kciid | ART002342033 | - |
dc.identifier.scopusid | 2-s2.0-85046705427 | - |
dc.citation.title | IMMUNE NETWORK | - |
dc.citation.volume | 18 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Kim, Hee Joo | - |
dc.contributor.affiliatedAuthor | Baek, Jinok | - |
dc.contributor.affiliatedAuthor | Lee, Jong Rok | - |
dc.contributor.affiliatedAuthor | Roh, Joo Young | - |
dc.contributor.affiliatedAuthor | Jung, YunJae | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Atopic dermatitis | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.subject.keywordAuthor | In vitro stimulation | - |
dc.subject.keywordPlus | EPITHELIUM-DERIVED CYTOKINES | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | HUMAN SKIN | - |
dc.subject.keywordPlus | IL-33 | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | FILAGGRIN | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | GAMMA | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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