Antitumor Effect of Calcium-Mediated Destabilization of Epithelial Growth Factor Receptor on Non-Small Cell Lung Carcinoma
DC Field | Value | Language |
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dc.contributor.author | Kim, In Un | - |
dc.contributor.author | Sung, In Sung | - |
dc.contributor.author | Sim, Jae Jun | - |
dc.contributor.author | Park, Minhee | - |
dc.contributor.author | Jeong, Keun-Yeong | - |
dc.contributor.author | Kim, Hwan Mook | - |
dc.date.available | 2020-02-27T11:41:19Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-04 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3918 | - |
dc.description.abstract | Despite the development of numerous therapeutics targeting the epithelial growth factor receptor (EGFR) for non-small cell lung carcinoma (NSCLC), the application of these drugs is limited because of drug resistance. Here, we investigated the antitumor effect of calcium-mediated degradation of EGFR pathway-associated proteins on NSCLC. First, lactate calcium salt (LCS) was utilized for calcium supplementation. Src, alpha-tubulin and EGFR levels were measured after LSC treatment, and the proteins were visualized by immunocytochemistry. Calpeptin was used to confirm the calcium-mediated effect of LCS on NSCLC. Nuclear expression of c-Myc and cyclin D1 was determined to understand the underlying mechanism of signal inhibition following EGFR and Src destabilization. The colony formation assay and a xenograft animal model were used to confirm the in vitro and in vivo antitumor effects, respectively. LCS supplementation reduced Src and alpha-tubulin expression in NSCLC cells. EGFR was destabilized because of proteolysis of Src and alpha-tubulin. c-Myc and cyclin D1 expression levels were also reduced following the decrease in the transcriptional co-activation of EGFR and Src. Clonogenic ability and tumor growth were significantly inhibited by LSC treatment-induced EGFR destabilization. These results suggest that other than specifically targeting EGFR, proteolysis of associated molecules such as Src or alpha-tubulin may effectively exert an antitumor effect on NSCLC via EGFR destabilization. Therefore, LCS is expected to be a good candidate for developing novel anti-NSCLC therapeutics overcoming chemoresistance. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.subject | COLORECTAL-CANCER CELLS | - |
dc.subject | TARGETED THERAPY | - |
dc.subject | CALPAIN | - |
dc.subject | PROTEIN | - |
dc.subject | EGFR | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | PROLIFERATION | - |
dc.subject | INACTIVATION | - |
dc.subject | TRAFFICKING | - |
dc.subject | HOMEOSTASIS | - |
dc.title | Antitumor Effect of Calcium-Mediated Destabilization of Epithelial Growth Factor Receptor on Non-Small Cell Lung Carcinoma | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000434978700237 | - |
dc.identifier.doi | 10.3390/ijms19041158 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.4 | - |
dc.identifier.scopusid | 2-s2.0-85045346017 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 19 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Kim, In Un | - |
dc.contributor.affiliatedAuthor | Sung, In Sung | - |
dc.contributor.affiliatedAuthor | Sim, Jae Jun | - |
dc.contributor.affiliatedAuthor | Kim, Hwan Mook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | non-small-cell lung carcinoma | - |
dc.subject.keywordAuthor | epithelial growth factor receptor | - |
dc.subject.keywordAuthor | Src | - |
dc.subject.keywordAuthor | alpha-tubulin | - |
dc.subject.keywordAuthor | calcium | - |
dc.subject.keywordAuthor | calpain | - |
dc.subject.keywordPlus | COLORECTAL-CANCER CELLS | - |
dc.subject.keywordPlus | TARGETED THERAPY | - |
dc.subject.keywordPlus | CALPAIN | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | EGFR | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INACTIVATION | - |
dc.subject.keywordPlus | TRAFFICKING | - |
dc.subject.keywordPlus | HOMEOSTASIS | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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