Anti-inflammatory and anti-arthritic effects of the ethanolic extract of Aralia continentalis Kitag. in IL-1 beta-stimulated human fibroblast-like synoviocytes and rodent models of polyarthritis and nociception
DC Field | Value | Language |
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dc.contributor.author | Hong, Riwon | - |
dc.contributor.author | Sur, BongJun | - |
dc.contributor.author | Yeom, Mijung | - |
dc.contributor.author | Lee, Bombi | - |
dc.contributor.author | Kim, Kyoung Soo | - |
dc.contributor.author | Rodriguez, Joyce P. | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Kang, Ki Sung | - |
dc.contributor.author | Huh, Chang-Ki | - |
dc.contributor.author | Lee, Sang Cheon | - |
dc.contributor.author | Hahm, Dae-Hyun | - |
dc.date.available | 2020-02-27T12:41:30Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-01-01 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/4179 | - |
dc.description.abstract | Background: Blocking the formation and invasive growth of pannus and its secretion of inflammatory cytokines and MMPs is important for treating rheumatoid arthritis. Hypothesis/Purpose: Anti-arthritic activity of Aralia continentalis Kitag., an oriental herbal medicine, and the underlying mechanisms involved were investigated. Study Design: Anti-inflammatory and anti-nocicpetive activities of the ethanolic extract (50% v/v) of Aralia continentalis Kitag. harvested from Imsil, Korea (ACI) were investigated in IL-1 beta-stimulated human fibroblast-like synoviocyte (FLS) cells and rodent models of collagen-induced polyarthritis and carrageenan-induced acute paw pain. Methods: In IL-1 beta-stimulated FLS cells derived from rheumatoid arthritis patients, the anti-inflammatory activity of ACI was examined by analyzing the expression levels of inflammatory mediators such as TNF-alpha, IL-6, IL-8, MMP-1, MMP-3, MMP-13, PGE2, and COX-2 using ELISA and RT-PCR analysis. The anti-arthritic activity of ACI was investigated by measuring body weight, squeaking score, paw volume, and arthritis index in collagen-induced polyarthritis mice. The anti-nociceptive activity of ACI was examined in the paw-pressure test and Tailflick latency test in rats. Results: The ethanolic extract (50% v/v) of ACI reduced the levels of TNF-alpha, IL-6, IL-8, MMP-1, and MMP-13 secreted by IL-1 beta-stimulated FLS cells, whereas MMP-3, COX-2, and PGE2 were not significantly affected. ACI inhibited the migration of NF-kappa B into the nucleus through the inhibition of ERK- and JNK-dependent MAP kinase pathways in IL-1 beta-stimulated FLS cells. In collagen-induced polyarthritis mice, oral administration of ACI extract (200 mg/kg) significantly alleviated arthritic behaviors. Histological observations of arthritic mouse knees were consistent with their behaviors. The anti-arthritic and anti-inflammatory activities of 200 mg/kg ACI extract were comparable to those of 10 mg/kg prednisolone when administered to mice. However, ACI administration did not significantly affect carrageenan-induced hyperalgesia or thermal nociception in rats. Conclusion: These results suggest that the ethanolic extract of ACI have significant anti-inflammatory and anti-arthritic effects in a rodent arthritis model and in IL-1 beta-stimulatedFLS cells. Thus, ACI may be a useful candidate for developing pharmaceuticals or dietary supplements for the treatment of inflammatory arthritis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER GMBH, URBAN & FISCHER VERLAG | - |
dc.relation.isPartOf | PHYTOMEDICINE | - |
dc.subject | N-TERMINAL KINASE | - |
dc.subject | INHIBITORY CONSTITUENTS | - |
dc.subject | CORDATA THUNB | - |
dc.subject | INFLAMMATORY ARTHRITIS | - |
dc.subject | CARTILAGE PROTECTION | - |
dc.subject | JOINT DESTRUCTION | - |
dc.subject | OSTEOARTHRITIS | - |
dc.subject | EXPRESSION | - |
dc.subject | MMP-13 | - |
dc.subject | ROOTS | - |
dc.title | Anti-inflammatory and anti-arthritic effects of the ethanolic extract of Aralia continentalis Kitag. in IL-1 beta-stimulated human fibroblast-like synoviocytes and rodent models of polyarthritis and nociception | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000425172900005 | - |
dc.identifier.doi | 10.1016/j.phymed.2017.10.016 | - |
dc.identifier.bibliographicCitation | PHYTOMEDICINE, v.38, pp.45 - 56 | - |
dc.identifier.scopusid | 2-s2.0-85032995869 | - |
dc.citation.endPage | 56 | - |
dc.citation.startPage | 45 | - |
dc.citation.title | PHYTOMEDICINE | - |
dc.citation.volume | 38 | - |
dc.contributor.affiliatedAuthor | Kang, Ki Sung | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Aralia continentalis Kitag. | - |
dc.subject.keywordAuthor | Rheumatoid arthritis | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Fibroblast-like synoviocyte | - |
dc.subject.keywordAuthor | Inflammatory cytokine | - |
dc.subject.keywordAuthor | Matrix metalloproteinase | - |
dc.subject.keywordPlus | N-TERMINAL KINASE | - |
dc.subject.keywordPlus | INHIBITORY CONSTITUENTS | - |
dc.subject.keywordPlus | CORDATA THUNB | - |
dc.subject.keywordPlus | INFLAMMATORY ARTHRITIS | - |
dc.subject.keywordPlus | CARTILAGE PROTECTION | - |
dc.subject.keywordPlus | JOINT DESTRUCTION | - |
dc.subject.keywordPlus | OSTEOARTHRITIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MMP-13 | - |
dc.subject.keywordPlus | ROOTS | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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