Effect of Trehalose and Erythritol on Post-thawed Miniature Pig Sperm
- Authors
- 김대영
- Issue Date
- 2018
- Publisher
- 한국키틴키토산학회
- Keywords
- Cryopreservation; Erythritol; Pig sperm; Reactive oxygen species; Trehalose
- Citation
- Journal of Chitin and Chitosan, v.23, no.1, pp.1 - 7
- Journal Title
- Journal of Chitin and Chitosan
- Volume
- 23
- Number
- 1
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5141
- DOI
- 10.17642/jcc.23.1.1
- ISSN
- 1229-4160
- Abstract
- Our objective was to evaluate the function of treahlose and erythritol in reducing reactive oxygen species concentrations, which is associated with a general improvement in the quality of frozen-thawing miniature pig sperm. Semen was mixed in modified Modena B extender, added to cooling media and freezing media, followed by the supplement of 100 mM trehalose and/or 100 mM erythritol with spermatozoa. The trehalose plus erythritol (TE) added group had less intracellular H2O2 than did control and trehalose (p<0.05). The percentage of viable acrosome-intact sperm (FITC-PNA-/PI-) was higher in erythritol and TE than controls (p<0.05 and p<0.001). The percentage of sperm with high fragmented DNA was observed in control group when compared with erythritol and TE also trehalose (p<0.001). The percentage of sperm lipid peroxidation was higher in control and trehalose than erythritol (p<0.01 and p<0.001), and was lowest in the TE (control and trehalose vs. TE: p<0.001, erythritol vs. TE: p<0.05). In conclusion, miniature pig sperm was successfully cryopreserved with trehalose plus erythritol provided the increasing the sperm quality and reducing the ROS.
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Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
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