Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action
DC Field | Value | Language |
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dc.contributor.author | Kim, Han Gyung | - |
dc.contributor.author | Choi, Subin | - |
dc.contributor.author | Lee, Jongsung | - |
dc.contributor.author | Hong, Yo Han | - |
dc.contributor.author | Jeong, Deok | - |
dc.contributor.author | Yoon, Keejung | - |
dc.contributor.author | Yoon, Deok Hyo | - |
dc.contributor.author | Sung, Gi-Ho | - |
dc.contributor.author | Lee, Seungihm | - |
dc.contributor.author | Hong, Suntaek | - |
dc.contributor.author | Yi, Young-Su | - |
dc.contributor.author | Kim, Jong-Hoon | - |
dc.contributor.author | Cho, Jae Youl | - |
dc.date.available | 2020-02-27T15:42:59Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 1741-427X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5222 | - |
dc.description.abstract | Celtis choseniana is the traditional plant used at Korea as a herbal medicine to ameliorate inflammatory responses. Although Celtis choseniana has been traditionally used as a herbal medicine at Korea, no systemic research has been conducted on its anti-inflammatory activity. Therefore, the present study explored an anti-inflammatory effect and its underlying molecular mechanism using Celtis choseniana methanol extract (Cc-ME) in macrophage-mediated inflammatory responses. In vitro anti-inflammatory activity of Cc-ME was evaluated using RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS), pam3CSK4 (Pam3), or poly(I:C). In vivo anti-inflammatory activity of Cc-ME was investigated using acute inflammatory disease mouse models, such as LPS-induced peritonitis and HCl/EtOH-induced gastritis. The molecular mechanism of Cc-ME-mediated anti-inflammatory activity was examined by Western blot analysis and immunoprecipitation using whole cell and nuclear fraction prepared from the LPS-stimulated RAW264.7 cells and HEK293 cells. Cc-ME inhibited NO production and mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and tumor necrosis factor-alpha (TNF-alpha) in the RAW264.7 cells and peritoneal macrophages induced by LPS, pam3, or poly(I:C) without cytotoxicity. High-performance liquid chromatography (HPLC) analysis showed that Cc-ME contained anti-inflammatory flavonoids quercetin, luteolin, and kaempferol. Among those, the content of luteolin, which showed an inhibitory effect on NO production, was highest. Cc-ME suppressed the NF-kappa B signaling pathway by targeting Src and interrupting molecular interactions between Src and p85, its downstream kinase. Moreover, Cc-ME ameliorated the morphological finding of peritonitis and gastritis in the mouse disease models. Therefore, these results suggest that Cc-ME exerted in vitro and in vivo anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory diseases. This anti-inflammatory activity of Cc-ME was dominantly mediated by targeting Src in NF-kappa B signaling pathway during macrophage-mediated inflammatory responses. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.relation.isPartOf | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.title | Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000428338700001 | - |
dc.identifier.doi | 10.1155/2018/3909038 | - |
dc.identifier.bibliographicCitation | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.2018 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85045047184 | - |
dc.citation.title | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.citation.volume | 2018 | - |
dc.contributor.affiliatedAuthor | Hong, Suntaek | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | QUERCETIN | - |
dc.subject.keywordPlus | KAEMPFEROL | - |
dc.subject.keywordPlus | HEPATITIS | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | GASTRITIS | - |
dc.subject.keywordPlus | SYMPTOMS | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | AP-1 | - |
dc.subject.keywordPlus | LPS | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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