Lespedeza bicolor ameliorates endothelial dysfunction induced by methylglyoxal glucotoxicity
DC Field | Value | Language |
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dc.contributor.author | Do, Moon Ho | - |
dc.contributor.author | Lee, Jae Hyuk | - |
dc.contributor.author | Wahedi, Hussain Mustatab | - |
dc.contributor.author | Pak, Chaeho | - |
dc.contributor.author | Lee, Choong Hwan | - |
dc.contributor.author | Yeo, Eui-Ju | - |
dc.contributor.author | Lim, Yunsook | - |
dc.contributor.author | Ha, Sang Keun | - |
dc.contributor.author | Choi, Inwook | - |
dc.contributor.author | Kim, Sun Yeou | - |
dc.date.available | 2020-02-27T16:41:19Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-12-01 | - |
dc.identifier.issn | 0944-7113 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5372 | - |
dc.description.abstract | Background: Lespedeza species have been used as a traditional medicine to treat nephritis, azotemia, inflammation, energy depletion, diabetes, and diuresis. Purpose: The purpose of this study is to screen the most potent Lespedeza species against methylglyoxal (MGO)-induced glucotoxicity, and to elucidate the mechanisms of action. Also, we will attempt to identify small chemical metabolites that might be responsible for such anti-glucotoxicity effects. Methods: Firstly, the protective effect of 26 different Lespedeza species against MGO-induced toxicity in human umbilical vein endothelial cells was investigated. The chemical metabolites of the most potent species (Lespedeza bicolor 1 (LB1) were identified by high pressure liquid chromatography quadrupole time-of-flight tandem mass spectrometry (HPLC-Q-TOF-MS/MS), then quantified by HPLC. The effects of LB1 on MGO-induced apoptosis were measured by annexin V-FITC staining and western blot. Inhibitory effects of LB1 on MGO-induced ROS generation, and effect of LB1 on advanced glycation end products (AGEs) inhibitor or a glycated cross-link breaker are also measured. Results: Among different Lespedeza species, LB1 extract was shown to reduce intracellular reactive oxidative species, exhibit anti-apoptotic effects, strongly inhibit all the mitogen-activated protein kinase signals, inhibit MGO-induced AGEs formation, and break down preformed AGEs. We tentatively identified 17 chemical constituents of LB1 by HPLC-Q-TOF-MS/MS. Among those, some components, such as genistein and quercetin, significantly reduced the AGEs formation and increased the AGEs-breaking activity, resulting in the reduction of glucotoxicity. Conclusion: LB1 extract has shown to be effective in preventing or treating MGO-induced endothelial dysfunction. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER GMBH, URBAN & FISCHER VERLAG | - |
dc.relation.isPartOf | PHYTOMEDICINE | - |
dc.subject | GLYCATION END-PRODUCTS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | TRAPPING METHYLGLYOXAL | - |
dc.subject | CELLS | - |
dc.subject | APOPTOSIS | - |
dc.subject | CYTOTOXICITY | - |
dc.subject | CONSTITUENTS | - |
dc.subject | INHIBITION | - |
dc.subject | MECHANISMS | - |
dc.subject | EXTRACTS | - |
dc.title | Lespedeza bicolor ameliorates endothelial dysfunction induced by methylglyoxal glucotoxicity | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000415693500004 | - |
dc.identifier.doi | 10.1016/j.phymed.2017.09.005 | - |
dc.identifier.bibliographicCitation | PHYTOMEDICINE, v.36, pp.26 - 36 | - |
dc.identifier.scopusid | 2-s2.0-85031002718 | - |
dc.citation.endPage | 36 | - |
dc.citation.startPage | 26 | - |
dc.citation.title | PHYTOMEDICINE | - |
dc.citation.volume | 36 | - |
dc.contributor.affiliatedAuthor | Do, Moon Ho | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyuk | - |
dc.contributor.affiliatedAuthor | Wahedi, Hussain Mustatab | - |
dc.contributor.affiliatedAuthor | Pak, Chaeho | - |
dc.contributor.affiliatedAuthor | Yeo, Eui-Ju | - |
dc.contributor.affiliatedAuthor | Kim, Sun Yeou | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Advanced glycation end products (AGEs) | - |
dc.subject.keywordAuthor | Methylglyoxal (MGO) | - |
dc.subject.keywordAuthor | Human umbilical vein endothelial cells | - |
dc.subject.keywordAuthor | Lespedeza bicolor (LB) | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Reactive oxygen species (ROS) | - |
dc.subject.keywordPlus | GLYCATION END-PRODUCTS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | TRAPPING METHYLGLYOXAL | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | CYTOTOXICITY | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | EXTRACTS | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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