Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro
DC Field | Value | Language |
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dc.contributor.author | Son, Eun Suk | - |
dc.contributor.author | Kim, Young Ock | - |
dc.contributor.author | Park, Chun Geon | - |
dc.contributor.author | Park, Kyung Hun | - |
dc.contributor.author | Jeong, Sung Hwan | - |
dc.contributor.author | Park, Jeong-Woong | - |
dc.contributor.author | Kim, Se-Hee | - |
dc.date.available | 2020-02-27T16:42:40Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-11-06 | - |
dc.identifier.issn | 1472-6882 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5469 | - |
dc.description.abstract | Background: Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf has been used in China as an herbal medicine. Many studies of this plant have reported anti-proliferative and apoptotic activities on human cancer cell lines. Therefore, this study of the anti-metastatic effect of Coix lacryma-jobi var. ma-yuen Stapf sprout extract (CLSE) in colorectal cancer cells may provide a scientific basis for exploring anti-cancer effects of edible crops. Methods: To evaluate the effect of CLSE on cell proliferation and signaling, we performed a Cell Counting Kit-8 (CCK-8) assay in HCT116 cells and used western blot analysis. Furthermore, scratch-wound healing, transwell migration, matrigel invasion, and adhesion assays were conducted to elucidate the anti-metastatic effects of CLSE under hypoxic conditions in colon cancer cells. Results: First, CLSE decreased deferoxamine (DFO)-induced migration of colon cancer cells by 87%, and blocked colon cancer cell migration by 80% compared with hypoxia control cells. Second, CLSE treatment resulted in a 54% reduction in hypoxia-induced invasiveness of colon cancer cells, and 50% inhibition of adhesive potency through inactivation of the extracellular signal-regulated kinase (ERK) 1/2 and protein kinase b (AKT) pathways. Third, conditioned medium collected from CLSE-treated HCT116 cells suppressed tube formation of human umbilical vein endothelial cells (HUVECs) by 91%. Conclusions: CLSE inhibited migration, invasion, and adhesion of colon cancer cells and tube formation by HUVECs via repression of the ERK1/2 and AKT pathways under hypoxic conditions. Therefore, CLSE may be used to treat patients with colon cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.relation.isPartOf | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.subject | LACHRYMA-JOBI | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | ADLAY SEED | - |
dc.subject | HYPOXIA | - |
dc.subject | METASTASIS | - |
dc.subject | IMPACT | - |
dc.subject | LUNG | - |
dc.title | Coix lacryma-jobi var. ma-yuen Stapf sprout extract has anti-metastatic activity in colon cancer cells in vitro | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000414678400002 | - |
dc.identifier.doi | 10.1186/s12906-017-1990-y | - |
dc.identifier.bibliographicCitation | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v.17 | - |
dc.identifier.scopusid | 2-s2.0-85032931336 | - |
dc.citation.title | BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | - |
dc.citation.volume | 17 | - |
dc.contributor.affiliatedAuthor | Son, Eun Suk | - |
dc.contributor.affiliatedAuthor | Jeong, Sung Hwan | - |
dc.contributor.affiliatedAuthor | Park, Jeong-Woong | - |
dc.contributor.affiliatedAuthor | Kim, Se-Hee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Coix | - |
dc.subject.keywordAuthor | Colon cancer | - |
dc.subject.keywordAuthor | Metastasis | - |
dc.subject.keywordAuthor | Invasion | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.subject.keywordPlus | LACHRYMA-JOBI | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | ADLAY SEED | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | LUNG | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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