Cynandione A from Cynanchum wilfordii Attenuates the Production of Inflammatory Mediators in LPS-Induced BV-2 Microglial Cells via NF-kappa B Inactivation
DC Field | Value | Language |
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dc.contributor.author | Yang, Seung Bo | - |
dc.contributor.author | Lee, Sang Min | - |
dc.contributor.author | Park, Ji-Hae | - |
dc.contributor.author | Lee, Tae Hoon | - |
dc.contributor.author | Baek, Nam-In | - |
dc.contributor.author | Park, Hi-Joon | - |
dc.contributor.author | Lee, Hyejung | - |
dc.contributor.author | Kim, Jiyoung | - |
dc.date.available | 2020-06-19T09:35:36Z | - |
dc.date.created | 2020-06-19 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/55167 | - |
dc.description.abstract | Cynanchum wilfordii is one of most widely used medicinal plants in Oriental medicine for the treatment of various conditions. In the present study, we isolated cynandione A (CA) from an extract of Cynanchum wilfordii roots (CWE) and investigated the effects of CA on the expression of inducible nitric oxide synthase (iNOS) and pro-inflammatory cytokines in lipopolysaccharide (LPS)-induced BV-2 microglial cells. CWE and CA significantly decreased LPS-induced nitric oxide production and the expression of iNOS in a concentration-dependent manner, while they (CWE up to 500 mu g/mL and CA up to 80 mu M) did not exhibit cytotoxic activity. Results from reverse transcription-polymerase chain reaction (RT-PCR) analysis and enzyme-linked immunosorbent assay (ELISA) showed that CA significantly attenuated the expression of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and IL-1 beta in LPS-stimulated BV-2 cells. Furthermore, CA inhibited the phosphorylation of inhibitor kappa B-alpha (I kappa B-alpha) and translocation of nuclear factor-kappa B (NF-kappa B) to the BV-2 cell nucleus, indicating that CWE and CA may have effective anti-inflammatory activities via NF-kappa B inactivation in stimulated microglial cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.relation.isPartOf | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.title | Cynandione A from Cynanchum wilfordii Attenuates the Production of Inflammatory Mediators in LPS-Induced BV-2 Microglial Cells via NF-kappa B Inactivation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000339693000018 | - |
dc.identifier.doi | 10.1248/bpb.b13-00939 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.37, no.8, pp.1390 - 1396 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84906724859 | - |
dc.citation.endPage | 1396 | - |
dc.citation.startPage | 1390 | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 37 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Yang, Seung Bo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cynanchum wilfordii | - |
dc.subject.keywordAuthor | natural product | - |
dc.subject.keywordAuthor | inflammatory cytokine | - |
dc.subject.keywordAuthor | inducible nitric oxide synthase (iNOS) | - |
dc.subject.keywordAuthor | nuclear factor-kappa B (NF-kappa B) | - |
dc.subject.keywordPlus | BETA-AMYLOID PROTEIN | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | NEURODEGENERATIVE DISEASES | - |
dc.subject.keywordPlus | ACTIVATED MICROGLIA | - |
dc.subject.keywordPlus | CONSTITUENTS | - |
dc.subject.keywordPlus | AURICULATUM | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | TOXICITY | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | RATS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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