Chemotaxis of Lipopolysaccharide-Stimulated RAW264.7 Macrophage Cell Line in Microfluidic Channels
DC Field | Value | Language |
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dc.contributor.author | Park, Junhyun | - |
dc.contributor.author | Koo, Jayoung | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | Chung, Kyung-Jin | - |
dc.contributor.author | Yi, Eun-Surk | - |
dc.contributor.author | Choi, Mi-Ri | - |
dc.contributor.author | Jung, YunJae | - |
dc.contributor.author | Cho, Sungbo | - |
dc.date.available | 2020-02-27T16:43:32Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5534 | - |
dc.description.abstract | Chemotaxis, the directed movement of cells in a gradient of chemoattractant, is essential for leukocyte migration under inflammatory conditions, but its accurate analysis is limited because of technical limitation. To investigate chemotactic activities of macrophage cell line, we stimulated RAW264.7 cells with a lipopolysaccharide (LPS) and observed the chemotactic migration of the stimulated RAW264.7 cells in the microfluidic channel. The microfluidic channel platform included the PDMS chamber and channel structure, which is 450 mu m long, 10 mu m wide, and 3 mu m high. The viability, surface phenotype, and production of nitric oxide of LPS-stimulated RAW264.7 cells were analyzed. The chemotactic migration number and speed of LPS-stimulated RAW264.7 cells to chemoattractant CCL5 in the microfluidic channels was evaluated. It was demonstrated that the microfluidic channel structure was feasible for analyzing the chemotactic migration of RAW264.7 macrophage cell line at the single cell level. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | MIGRATION | - |
dc.subject | PLATFORM | - |
dc.subject | ASSAY | - |
dc.title | Chemotaxis of Lipopolysaccharide-Stimulated RAW264.7 Macrophage Cell Line in Microfluidic Channels | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000414491600031 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.17, no.11, pp.7996 - 8000 | - |
dc.identifier.scopusid | 2-s2.0-85027332625 | - |
dc.citation.endPage | 8000 | - |
dc.citation.startPage | 7996 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Park, Junhyun | - |
dc.contributor.affiliatedAuthor | Koo, Jayoung | - |
dc.contributor.affiliatedAuthor | Kim, Ji-Youn | - |
dc.contributor.affiliatedAuthor | Chung, Kyung-Jin | - |
dc.contributor.affiliatedAuthor | Yi, Eun-Surk | - |
dc.contributor.affiliatedAuthor | Choi, Mi-Ri | - |
dc.contributor.affiliatedAuthor | Jung, YunJae | - |
dc.contributor.affiliatedAuthor | Cho, Sungbo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Cell Migration | - |
dc.subject.keywordAuthor | Chemoattractant | - |
dc.subject.keywordAuthor | Migration Speed | - |
dc.subject.keywordAuthor | PDMS | - |
dc.subject.keywordAuthor | RAW264.7 Cells | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | ASSAY | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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