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Fluorescence Quenching and Enhancement of DNA-Templated Silver Nanocluster by DNA Hybridization
- Authors
- Lee, Chunghyun; Gang, Jongback
- Issue Date
- Nov-2017
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- DNA-Templated Silver Nanocluster; Fluorescence Quenching; Electron Transfer; Mismatched Base
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.17, no.11, pp.7961 - 7965
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 17
- Number
- 11
- Start Page
- 7961
- End Page
- 7965
- ISSN
- 1533-4880
- Abstract
- Oligonucleotide-templated Ag nanoclusters (DNA-AgNCs) has been widely used to detect biomolecules of interest due to the useful properties. To understand fluorescence quenching and enhancement of DNA-AgNCs by cDNA, this study used cDNA containing several mismatched bases (cDNA-1) and the full-length cDNA with a mismatched base (f-cDNA-1). We showed that DNA-AgNCs decreased fluorescence intensities to different degrees by hybridization of cDNA and cDNA-1. However, the fluorescence intensities of DNA-AgNCs in the presence of f-cDNA and f-cDNA-1 increased by 153% and 491%, respectively, compared to that of DNA-AgNCs, respectively.
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Related Researcher
- Gang, Jong Back
- BioNano Technology (Department of Chemistry)