Laboratory diagnosis of Clostridium difficile infection: Comparison of Techlab C. diff Quik Chek Complete, Xpert C. difficile, and multistep algorithmic approach
DC Field | Value | Language |
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dc.contributor.author | Seo, Ja Young | - |
dc.contributor.author | Jeong, Ji Hun | - |
dc.contributor.author | Kim, Kyung Hee | - |
dc.contributor.author | Ahn, Jeong-Yeal | - |
dc.contributor.author | Park, Pil-Whan | - |
dc.contributor.author | Seo, Yiel-Hea | - |
dc.date.available | 2020-02-27T16:43:42Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-11 | - |
dc.identifier.issn | 0887-8013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5547 | - |
dc.description.abstract | BackgroundClostridium difficile is a major pathogen responsible for nosocomial infectious diarrhea. We explored optimal laboratory strategies for diagnosis of C.difficile infection (CDI) in our clinical settings, a 1400-bed tertiary care hospital. MethodsUsing 191 fresh stool samples from adult patients, we evaluated the performance of Xpert C. difficile (Xpert CD), C. diff Quik Chek Complete (which simultaneously detects glutamate dehydrogenase [GDH] and C.difficile toxins [CDT]), toxigenic culture, and a two-step algorithm composed of GDH/CDT as a screening test and Xpert CD as a confirmatory test. ResultsClostridium difficile was detected in 35 samples (18.3%), and all isolates were toxigenic strains. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value of each assay for detecting CDI were as follows: Quik Chek Complete CDT (45.7%, 100%, 100%, 89.1%), Quik Chek Complete GDH (97.1%, 99.4%, 97.1%, 99.4%), Xpert CD (94.3%, 100%, 100%, 98.7%), and toxigenic culture (91.4%, 100%, 100%, 98.1%). A two-step algorithm performed identically with Xpert CD assay. ConclusionOur data showed that most C.difficile isolates from adult patients were toxigenic. We demonstrated that a two-step algorithm based on GDH/CDT assay followed by Xpert CD assay as a confirmatory test was rapid, reliable, and cost effective for diagnosis of CDI in an adult patient setting with high prevalence of toxigenic C.difficile. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | JOURNAL OF CLINICAL LABORATORY ANALYSIS | - |
dc.subject | TOXIN-B | - |
dc.subject | GLUTAMATE-DEHYDROGENASE | - |
dc.subject | ENZYME-IMMUNOASSAY | - |
dc.subject | TESTING ALGORITHMS | - |
dc.subject | CYTOTOXIN ASSAY | - |
dc.subject | RAPID DETECTION | - |
dc.subject | CULTURE | - |
dc.subject | DIARRHEA | - |
dc.subject | ANTIGEN | - |
dc.subject | EPIDEMIOLOGY | - |
dc.title | Laboratory diagnosis of Clostridium difficile infection: Comparison of Techlab C. diff Quik Chek Complete, Xpert C. difficile, and multistep algorithmic approach | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000416049100024 | - |
dc.identifier.doi | 10.1002/jcla.22135 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL LABORATORY ANALYSIS, v.31, no.6 | - |
dc.identifier.scopusid | 2-s2.0-85013103863 | - |
dc.citation.title | JOURNAL OF CLINICAL LABORATORY ANALYSIS | - |
dc.citation.volume | 31 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Seo, Ja Young | - |
dc.contributor.affiliatedAuthor | Jeong, Ji Hun | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hee | - |
dc.contributor.affiliatedAuthor | Ahn, Jeong-Yeal | - |
dc.contributor.affiliatedAuthor | Park, Pil-Whan | - |
dc.contributor.affiliatedAuthor | Seo, Yiel-Hea | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | adult | - |
dc.subject.keywordAuthor | Clostridium difficile | - |
dc.subject.keywordAuthor | diagnosis | - |
dc.subject.keywordAuthor | glutamate dehydrogenase | - |
dc.subject.keywordAuthor | multistep algorithm | - |
dc.subject.keywordAuthor | tertiary care hospital | - |
dc.subject.keywordPlus | TOXIN-B | - |
dc.subject.keywordPlus | GLUTAMATE-DEHYDROGENASE | - |
dc.subject.keywordPlus | ENZYME-IMMUNOASSAY | - |
dc.subject.keywordPlus | TESTING ALGORITHMS | - |
dc.subject.keywordPlus | CYTOTOXIN ASSAY | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DIARRHEA | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | EPIDEMIOLOGY | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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