RANKL-induced osteoclastogenesis is suppressed by 4-O-methylhonokiol in bone marrow-derived macrophages
DC Field | Value | Language |
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dc.contributor.author | Park, Kyung-Ran | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | Kim, Eun-Cheol | - |
dc.contributor.author | Yun, Hyung-Mun | - |
dc.contributor.author | Hong, Jin Tae | - |
dc.date.available | 2020-02-27T17:44:03Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 0253-6269 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5852 | - |
dc.description.abstract | Magnolol, honokiol, and obovatol are well known bioactive constituents of the bark of Magnolia officinalis and have been reported to have beneficial effects in various diseases. We recently isolated a novel active compound, 4-O-methylhonokiol (4-O-MH) from the ethanol extract of M. officinalis, which was previously reported to have pharmacological effects including anti-inflammatory, anti-oxidative, and anti-aging activities. Here, we examined the pharmacological properties of 4-O-MH on osteoblast (bone-forming cells) and osteoclast (bone-resorbing cells) differentiation, and its underlying signaling pathways in primary cultured pre-osteoblasts and bone marrow macrophages. Our results showed that 4-O-MH did not affect cell viability in pre-osteoblasts and did not influence osteoblast differentiation and mineralized nodule formation, as assessed by alkaline phosphatase activity and Alizarin red staining. However, 4-O-MH significantly inhibited TRAP-positive multinuclear osteoclasts and F-actin ring formation during Receptor activator of NF-kappa B ligand (RANKL)-mediated osteoclastogenesis without cytotoxicity. In addition, 4-O-MH suppressed RANKL-induced critical factors (c-Fos, NF-ATc1, TRAP, and ITB3) for osteoclast differentiation and function. Furthermore, RANKL-mediated signaling, including ERK1/2, AKT, and NF-kB pathways was attenuated by 4-O-MH. Taken together, 4-O-MH has an inhibitory role in RANKL-mediated osteoclastogenesis but not osteoblast differentiation, and our findings also suggest that 4-O-MH is a potential therapeutic agent for bone-destructive diseases such as osteoporosis, alveolar bone resorption, and osteoarthritis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PHARMACEUTICAL SOC KOREA | - |
dc.relation.isPartOf | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.subject | OSTEOBLASTIC DIFFERENTIATION | - |
dc.subject | MC3T3-E1 CELLS | - |
dc.subject | C-FOS | - |
dc.subject | OSTEOPOROSIS | - |
dc.subject | ACTIVATION | - |
dc.subject | RECEPTOR | - |
dc.subject | MINERALIZATION | - |
dc.subject | RESORPTION | - |
dc.subject | PATHWAYS | - |
dc.subject | SYSTEM | - |
dc.title | RANKL-induced osteoclastogenesis is suppressed by 4-O-methylhonokiol in bone marrow-derived macrophages | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000408766200006 | - |
dc.identifier.doi | 10.1007/s12272-017-0932-z | - |
dc.identifier.bibliographicCitation | ARCHIVES OF PHARMACAL RESEARCH, v.40, no.8, pp.933 - 942 | - |
dc.identifier.kciid | ART002257122 | - |
dc.identifier.scopusid | 2-s2.0-85025447755 | - |
dc.citation.endPage | 942 | - |
dc.citation.startPage | 933 | - |
dc.citation.title | ARCHIVES OF PHARMACAL RESEARCH | - |
dc.citation.volume | 40 | - |
dc.citation.number | 8 | - |
dc.contributor.affiliatedAuthor | Kim, Ji-Youn | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Magnolia officinalis | - |
dc.subject.keywordAuthor | 4-O-methylhonokiol | - |
dc.subject.keywordAuthor | Osteoblast | - |
dc.subject.keywordAuthor | Osteoclast | - |
dc.subject.keywordAuthor | RANKL | - |
dc.subject.keywordPlus | OSTEOBLASTIC DIFFERENTIATION | - |
dc.subject.keywordPlus | MC3T3-E1 CELLS | - |
dc.subject.keywordPlus | C-FOS | - |
dc.subject.keywordPlus | OSTEOPOROSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | MINERALIZATION | - |
dc.subject.keywordPlus | RESORPTION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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