Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis
DC Field | Value | Language |
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dc.contributor.author | Jang, Young-Rock | - |
dc.contributor.author | Shin, Yong | - |
dc.contributor.author | Jin, Choong Eun | - |
dc.contributor.author | Koo, Bonhan | - |
dc.contributor.author | Park, Se Yoon | - |
dc.contributor.author | Kim, Min-Chul | - |
dc.contributor.author | Kim, Taeeun | - |
dc.contributor.author | Chong, Yong Pil | - |
dc.contributor.author | Lee, Sang-Oh | - |
dc.contributor.author | Choi, Sang-Ho | - |
dc.contributor.author | Kim, Yang Soo | - |
dc.contributor.author | Woo, Jun Hee | - |
dc.contributor.author | Kim, Sung-Han | - |
dc.contributor.author | Yu, Eunsil | - |
dc.date.available | 2020-02-27T17:44:43Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-07-03 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5905 | - |
dc.description.abstract | Background Serologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalinfixed liver tissues in Q fever patients with acute hepatitis. Methods We reviewed the clinical and laboratory data in patients with Q fever hepatitis who underwent liver biopsy during a 17-year period, and whose biopsied tissues were available. We also selected patients who revealed granuloma in liver biopsy and with no Q fever diagnosis within the last 3 years as control. Acute Q fever hepatitis was diagnosed if two or more of the following clinical, serologic, or histopathologic criteria were met: (1) an infectious hepatitislike clinical feature such as fever (>= 38 degrees C) with elevated hepatic transaminase levels; (2) exhibition of a phase II immunoglobulin G (IgG) antibodies titer by IFA of >= 1:128 in single determination, or a four-fold or greater rise between two separate samples obtained two or more weeks apart; (3) histologic finding of biopsy tissue showing characteristic fibrin ring granuloma. Results A total of 11 patients with acute Q fever hepatitis were selected and analyzed. Of the 11 patients, 3 (27%) had exposure to zoonotic risk factors and 7 (63%) met the serologic criteria. Granulomas with either circumferential or radiating fibrin deposition were observed in 10 cases on liver biopsy and in 1 case on bone marrow biopsy. 8 (73%) revealed positive Coxiella burnetii PCR from their formalin-fixed liver tissues. In contrast, none of 10 patients with alternative diagnosis who had hepatic granuloma revealed positive C. burnetii PCR from their formalin-fixed liver tissues. Conclusions Q fever PCR from formalin-fixed liver tissues appears to be a useful adjunct for diagnosing Q fever hepatitis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.subject | LONG-TERM PERSISTENCE | - |
dc.subject | DIAGNOSIS | - |
dc.subject | INFECTION | - |
dc.subject | ORIGIN | - |
dc.title | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000405268500032 | - |
dc.identifier.doi | 10.1371/journal.pone.0180237 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.12, no.7 | - |
dc.identifier.scopusid | 2-s2.0-85021680470 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 12 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Jang, Young-Rock | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | LONG-TERM PERSISTENCE | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | ORIGIN | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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