Visual determination of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of magnetic nanoparticles functionalized with a poly(ethylene glycol) derivative
DC Field | Value | Language |
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dc.contributor.author | Shin, Ho Yun | - |
dc.contributor.author | Kim, Bong-Geun | - |
dc.contributor.author | Cho, Seongyeon | - |
dc.contributor.author | Lee, Jongwon | - |
dc.contributor.author | Na, Hyon Bin | - |
dc.contributor.author | Kim, Moon Il | - |
dc.date.available | 2020-02-27T18:40:59Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 0026-3672 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/5934 | - |
dc.description.abstract | A colorimetric assay is described for the determination of H2O2 and glucose based on the peroxidase-mimicking activity of Fe3O4 magnetic nanoparticles functionalized with a poly(ethylene glycol) derivative (PEG-MNPs). PEG ligands conjugated to a phosphine oxide and a phospholipid were synthesized and deposited on the surface of magnetic nanoparticles (MNPs). This leads to enhanced peroxidase-like activity of the MNPs by providing more monodispersed MNPs to be present in solution and by reducing self-aggregation. The PEG-MNPs were then used as enzyme mimetics that catalyze the oxidation of 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) to form a green product having an absorption maximum at 417 nm which enables a visual detection of H2O2. These findings are exploited in a highly sensitive and selective glucose bioassay based on the formation of H2O2 by glucose oxidase (GOx). The calibration plot covers the glucose concentration range from 5 mu M to 1 mM, and the limit of detection is as low as 3 mu M. The diagnostic capability of the method is demonstrated by analyzing glucose in human blood serum. It is perceived that the PEG-MNPs presented here also may act as potent peroxidase mimetics in other methods for the determination of clinically important analytes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER WIEN | - |
dc.relation.isPartOf | MICROCHIMICA ACTA | - |
dc.subject | COLORIMETRIC DETECTION | - |
dc.subject | OXIDASE | - |
dc.subject | NANOCOMPOSITE | - |
dc.subject | PROTEIN | - |
dc.subject | H2O2 | - |
dc.title | Visual determination of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of magnetic nanoparticles functionalized with a poly(ethylene glycol) derivative | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000403353000026 | - |
dc.identifier.doi | 10.1007/s00604-017-2198-z | - |
dc.identifier.bibliographicCitation | MICROCHIMICA ACTA, v.184, no.7, pp.2115 - 2122 | - |
dc.identifier.scopusid | 2-s2.0-85017128270 | - |
dc.citation.endPage | 2122 | - |
dc.citation.startPage | 2115 | - |
dc.citation.title | MICROCHIMICA ACTA | - |
dc.citation.volume | 184 | - |
dc.citation.number | 7 | - |
dc.contributor.affiliatedAuthor | Cho, Seongyeon | - |
dc.contributor.affiliatedAuthor | Kim, Moon Il | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Colorimetric biosensor | - |
dc.subject.keywordAuthor | Peroxidase mimics | - |
dc.subject.keywordAuthor | Glucose detection | - |
dc.subject.keywordAuthor | Monodispersed magnetic nanoparticles | - |
dc.subject.keywordAuthor | Surface coating | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | OXIDASE | - |
dc.subject.keywordPlus | NANOCOMPOSITE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | H2O2 | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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