A novel CMOS image sensor system for quantitative loop-mediated isothermal amplification assays to detect food-borne pathogens
DC Field | Value | Language |
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dc.contributor.author | Wang, Tiantian | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.contributor.author | An, Jeong Ho | - |
dc.date.available | 2020-02-27T19:44:25Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6464 | - |
dc.description.abstract | Loop-mediated isothermal amplification (LAMP) is considered as one of the alternatives to the conventional PCR and it is an inexpensive portable diagnostic system with minimal power consumption. The present work describes the application of LAMP in real-time photon detection and quantitative analysis of nucleic acids integrated with a disposable complementary-metal-oxide semiconductor (CMOS) image sensor. This novel system works as an amplification-coupled detection platform, relying on a CMOS image sensor, with the aid of a computerized circuitry controller for the temperature and light sources. The CMOS image sensor captures the light which is passing through the sensor surface and converts into digital units using an analog-to-digital converter (ADC). This new system monitors the real-time photon variation, caused by the color changes during amplification. Escherichia coli 0157 was used as a proof-of-concept target for quantitative analysis, and compared with the results for Staphylococcus aureus and Salmonella enterica to confirm the efficiency of the system. The system detected various DNA concentrations of E. coli 0157 in a short time (45 min), with a detection limit of 10 fg/mu L. The low-cost, simple, and compact design, with low power consumption, represents a significant advance in the development of a portable, sensitive, user-friendly, real-time, and quantitative analytic tools for point-of-care diagnosis. (C) 2016 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.subject | OF-CARE DIAGNOSTICS | - |
dc.subject | DNA AMPLIFICATION | - |
dc.subject | CHIP | - |
dc.subject | BACTERIA | - |
dc.subject | SAMPLES | - |
dc.subject | LAMP | - |
dc.title | A novel CMOS image sensor system for quantitative loop-mediated isothermal amplification assays to detect food-borne pathogens | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000394197200001 | - |
dc.identifier.doi | 10.1016/j.mimet.2016.12.002 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGICAL METHODS, v.133, pp.1 - 7 | - |
dc.identifier.scopusid | 2-s2.0-85006102825 | - |
dc.citation.endPage | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.citation.volume | 133 | - |
dc.contributor.affiliatedAuthor | Wang, Tiantian | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | CMOS-based LAMP system | - |
dc.subject.keywordAuthor | Molecular diagnosis | - |
dc.subject.keywordAuthor | Photon count variation | - |
dc.subject.keywordAuthor | Quantitative analysis | - |
dc.subject.keywordPlus | OF-CARE DIAGNOSTICS | - |
dc.subject.keywordPlus | DNA AMPLIFICATION | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | SAMPLES | - |
dc.subject.keywordPlus | LAMP | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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