ANTI-INFLAMMATORY EFFECTS OF SIMVASTATIN IN NONSTEROIDAL ANTI-INFLAMMATORY DRUGS-INDUCED SMALL BOWEL INJURY
DC Field | Value | Language |
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dc.contributor.author | Kim, E. K. | - |
dc.contributor.author | Cho, J. H. | - |
dc.contributor.author | Jeong, A. R. | - |
dc.contributor.author | Kim, E. J. | - |
dc.contributor.author | Park, D. K. | - |
dc.contributor.author | Kwon, K. A. | - |
dc.contributor.author | Chung, J. W. | - |
dc.contributor.author | Kim, K. O. | - |
dc.contributor.author | Kim, J. H. | - |
dc.contributor.author | Kim, J. H. | - |
dc.contributor.author | Kim, Y. J. | - |
dc.date.available | 2020-02-27T19:44:32Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 0867-5910 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6477 | - |
dc.description.abstract | Small bowel injury can occur as the result of a multifaceted process that includes increased acid secretion, generation of reactive oxygen species, and cyclooxygenase inhibition. However, no effective medication for small bowel ulceration is available. Simvastatin is an important lipid-lowering agent with anti-inflammatory activity. We aimed to validate the effects of simvastatin in vitro and in vivo. In presence or absence of simvastatin, IEC-6 small bowel cell line with 50 ng/ml of tumor nectosis factor alpha (TNF-alpha) was investigated by western blotting, qRT-PCR, and DCF-DA assay. In addition, an in vivo study of nonsteroidal anti-inflammatory drugs (NSAID)-induced small bowel inflammation was performed using 7-week-old specific-pathogen-free (SPF) male C57BL/6 mice. Simvastatin treatment reduced the mRNA levels of interleukin-6 and interleukin-8 by approximately 50% in TNF-alpha-stimulated IEC-6 cells. Treatment with a combination of 50 ng/ml TNF-alpha and 0.5 mu M simvastatin decreased activation of Akt, I kappa B alpha, and nuclear factor-kappa B p65 level in IEC-6 cells. By DCF-DA staining, intracellular reactive oxygen species (ROS) production was increased in TNF-a-stimulated cells, and treatment with simvastatin decreased the level of ROS. In addition, in vivo mouse model of NSAID-induced small bowel inflammation, the administration of simvastatin reduced the number of small bowel hemorrhagic lesions and the level of ROS production as determined by gross examination and 8-hydroxydeoxyguanosine immunohistochemistry of small bowel tissue, respectively. Simvastatin reduced NSAID-induced injuries by both suppression of ROS generation and modulation of inflammatory cytokines in vitro and in vivo. Therefore, simvastatin, an HMG-CoA reductase inhibitor, has potential as a prophylactic and therapeutic agent for NSAID-induced small bowel injury. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | POLISH PHYSIOLOGICAL SOC | - |
dc.relation.isPartOf | JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | - |
dc.subject | NECROSIS-FACTOR-ALPHA | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | HELICOBACTER-PYLORI | - |
dc.subject | DAMAGE | - |
dc.subject | ULCER | - |
dc.subject | INFORMATION | - |
dc.subject | MEDIATORS | - |
dc.subject | INTESTINE | - |
dc.subject | PROTEINS | - |
dc.subject | ETIOLOGY | - |
dc.title | ANTI-INFLAMMATORY EFFECTS OF SIMVASTATIN IN NONSTEROIDAL ANTI-INFLAMMATORY DRUGS-INDUCED SMALL BOWEL INJURY | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000401206800007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, v.68, no.1, pp.69 - 77 | - |
dc.identifier.scopusid | 2-s2.0-85018297783 | - |
dc.citation.endPage | 77 | - |
dc.citation.startPage | 69 | - |
dc.citation.title | JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | - |
dc.citation.volume | 68 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Kim, E. K. | - |
dc.contributor.affiliatedAuthor | Cho, J. H. | - |
dc.contributor.affiliatedAuthor | Jeong, A. R. | - |
dc.contributor.affiliatedAuthor | Kim, E. J. | - |
dc.contributor.affiliatedAuthor | Park, D. K. | - |
dc.contributor.affiliatedAuthor | Kwon, K. A. | - |
dc.contributor.affiliatedAuthor | Chung, J. W. | - |
dc.contributor.affiliatedAuthor | Kim, K. O. | - |
dc.contributor.affiliatedAuthor | Kim, J. H. | - |
dc.contributor.affiliatedAuthor | Kim, J. H. | - |
dc.contributor.affiliatedAuthor | Kim, Y. J. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | 3-hydroxy-3methylglutatyl-coenzyme A | - |
dc.subject.keywordAuthor | reductase inhibitor | - |
dc.subject.keywordAuthor | simvastatin | - |
dc.subject.keywordAuthor | non-steroidal anti-inflammatory drugs | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | tumor necrosis factor-a | - |
dc.subject.keywordAuthor | interleukin-6 | - |
dc.subject.keywordAuthor | interleukin-8 | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | ULCER | - |
dc.subject.keywordPlus | INFORMATION | - |
dc.subject.keywordPlus | MEDIATORS | - |
dc.subject.keywordPlus | INTESTINE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ETIOLOGY | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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