Synergistically Anti-metastatic Effect of 5-Flourouracil on Colorectal Cancer Cells via Calcium-mediated Focal Adhesion Kinase Proteolysis
DC Field | Value | Language |
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dc.contributor.author | Park, Minhee | - |
dc.contributor.author | Sundaramoorthy, Pasupathi | - |
dc.contributor.author | Sim, Jae Jun | - |
dc.contributor.author | Jeong, Keun-Yeong | - |
dc.contributor.author | Kim, Hwan Mook | - |
dc.date.available | 2020-02-27T19:45:09Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6516 | - |
dc.description.abstract | Aim: To investigate the possibility of enhancing an anti-metastatic effect of 5-fluorouracil (5-FU) on colorectal cancer (CRC) cells by combining it with continuous calcium supplementation. Materials and Methods: Optimal doses of 5-FU with/without lactate salt (CaLa) were determined via clonogenicity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assays using human CRC cells cultured on normal or low-attachment plates. Invasion and migration assays confirmed the enhanced anti-metastatic effect of combining 5-FU and CaLa. Western blot analysis for elements of the focal adhesion kinase (FAK) signaling cascade and epithelial-mesenchymal transition (EMT) markers was used to investigate the underlying mechanism. Results: 5-FU (2.5 mu M) had no antitumor activity against unanchored CRC cells, while it significantly suppressed anchorage-dependent cell proliferation. In contrast, treatment with CaLa (2.5 mM), alone and in combination with 5-FU, exerted antitumor activity against both anchored and unanchored CRC cells via calcium-mediated FAK proteolysis and inhibition of EMT markers, such as vimentin and SNAIL. Conclusion: Calcium supplementation represents a method of enhancing the potency of existing antitumor agents such as 5-FU, augmenting their clinical effectiveness. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | INT INST ANTICANCER RESEARCH | - |
dc.relation.isPartOf | ANTICANCER RESEARCH | - |
dc.subject | MECHANISMS | - |
dc.subject | ANOIKIS | - |
dc.subject | STATE | - |
dc.subject | FAK | - |
dc.title | Synergistically Anti-metastatic Effect of 5-Flourouracil on Colorectal Cancer Cells via Calcium-mediated Focal Adhesion Kinase Proteolysis | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000391958800014 | - |
dc.identifier.doi | 10.21873/anticanres.11295 | - |
dc.identifier.bibliographicCitation | ANTICANCER RESEARCH, v.37, no.1, pp.103 - 114 | - |
dc.identifier.scopusid | 2-s2.0-85007566289 | - |
dc.citation.endPage | 114 | - |
dc.citation.startPage | 103 | - |
dc.citation.title | ANTICANCER RESEARCH | - |
dc.citation.volume | 37 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Park, Minhee | - |
dc.contributor.affiliatedAuthor | Sundaramoorthy, Pasupathi | - |
dc.contributor.affiliatedAuthor | Sim, Jae Jun | - |
dc.contributor.affiliatedAuthor | Kim, Hwan Mook | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Colorectal cancer | - |
dc.subject.keywordAuthor | metastasis | - |
dc.subject.keywordAuthor | 5-FU | - |
dc.subject.keywordAuthor | lactate calcium salt | - |
dc.subject.keywordAuthor | focal adhesion kinase | - |
dc.subject.keywordAuthor | epithelial-mesenchymal transition | - |
dc.subject.keywordAuthor | combination treatment | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | ANOIKIS | - |
dc.subject.keywordPlus | STATE | - |
dc.subject.keywordPlus | FAK | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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