Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine
DC Field | Value | Language |
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dc.contributor.author | Lee, Sang Weon | - |
dc.contributor.author | Kim, Sung Jin | - |
dc.contributor.author | Kim, Hyungwoo | - |
dc.contributor.author | Yang, Dongki | - |
dc.contributor.author | Kim, Hyun Jung | - |
dc.contributor.author | Kim, Byung Joo | - |
dc.date.available | 2020-02-27T19:45:19Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 1792-0981 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6525 | - |
dc.description.abstract | Interstitial cells of Cajal (ICCs) function as pacemaker cells in the gastrointestinal (GI) tract and therefore, serve an important role in regulating GI motility. The effects of a species of plum (Prunus mume Siebold & Zucc.) on cultured ICC cluster-induced pacemaker potentials in the mouse small intestine were investigated, and the effects of a methanolic extract of Prunus mume (m-PM) on ICC pacemaker activities were examined using the whole-cell patch-clamp technique. ICC pacemaker membrane potentials were depolarized by m-PM in a concentration dependent manner in current clamp mode. 4-Diphenylacetoxy-N-methyl-piperidine methiodide, which is a muscarinic 3 (M-3) receptor antagonist, was able to block m-PM-induced pacemaker potential increases, whereas methoctramine, which is a muscarinic 2 (M-2) receptor antagonist, was not. When 1 mM guanosine diphosphate (3-5 was present in the pipette solution, m-PM induced slight pacemaker depolarization. Following pretreatment in bath solution of Ca2+-free solution or a Ca'+-ATPase inhibitor in endoplasmic reticulum, the pacemaker currents were inhibited. Furthermore, pretreatment with PD98059, SB203580 or SP600125, which is a c-jun NH2-terminal kinase inhibitor, blocked m-PM-induced ICC potential depolarization. Furthermore, m-PM inhibited transient receptor potential melastatin (TRPM) 7 channels, but did not affect Ca2+-activated Cl- channels. These results suggest that m-PM is able to modulate pacemaker potentials through the muscarinic M-3 receptor, via G-protein and external and internal Ca2+, in a mitogen-activated protein kinase and TRPM7-dependent manner. Therefore, m-PM may provide a basis for the development of a novel gastroprokinetic agent. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.relation.isPartOf | EXPERIMENTAL AND THERAPEUTIC MEDICINE | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | GTP-BINDING PROTEIN | - |
dc.subject | IN-VITRO | - |
dc.subject | GASTROINTESTINAL-TRACT | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | CANCER-CELLS | - |
dc.subject | TNF-ALPHA | - |
dc.subject | CURRENTS | - |
dc.subject | CONDUCTANCE | - |
dc.subject | CHANNELS | - |
dc.title | Effects of Prunus mume Siebold & Zucc. in the pacemaking activity of interstitial cells of Cajal in murine small intestine | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000393018600053 | - |
dc.identifier.doi | 10.3892/etm.2016.3963 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND THERAPEUTIC MEDICINE, v.13, no.1, pp.327 - 334 | - |
dc.identifier.scopusid | 2-s2.0-85005949239 | - |
dc.citation.endPage | 334 | - |
dc.citation.startPage | 327 | - |
dc.citation.title | EXPERIMENTAL AND THERAPEUTIC MEDICINE | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Yang, Dongki | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | interstitial cells of Cajal | - |
dc.subject.keywordAuthor | plum | - |
dc.subject.keywordAuthor | Prunus mume Siebold & | - |
dc.subject.keywordAuthor | Zucc. | - |
dc.subject.keywordAuthor | gastrointestinal tract | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | GTP-BINDING PROTEIN | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | GASTROINTESTINAL-TRACT | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | CURRENTS | - |
dc.subject.keywordPlus | CONDUCTANCE | - |
dc.subject.keywordPlus | CHANNELS | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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