Naringenin causes ASK1-induced apoptosis via reactive oxygen species in human pancreatic cancer cells
DC Field | Value | Language |
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dc.contributor.author | Park, Hyun Jin | - |
dc.contributor.author | Choi, Yong Jun | - |
dc.contributor.author | Lee, Jeong Hyun | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.available | 2020-02-27T20:41:28Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-01 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/6574 | - |
dc.description.abstract | Naringenin, one of the most abundant flavonoids in natural citrus fruits, has been investigated for its ability to inhibit growth of breast, colon, gastric and prostate cancer cells. However, naringenin-induced cell death in pancreatic cancer is not well understood. Therefore, we analyzed the naringenin-induced apoptosis mechanism using human pancreatic cancer SNU-213 cells. Annexin V+/PI + marked cells increased from 5.10% to 8.29%, 25.06% and 35.31% in response to treatment with 200, 400, and 600 mu M naringenin, respectively. Two-dimensional electrophoresis to identify possible target-related proteins of naringenin-induced apoptosis revealed seven proteins. Among these, the expression of peroxiredoxin-1 (Prdx-1), which modulates redox homeostasis of cells, was decreased. To obtain a broad understanding of the interactive mechanism of naringenin and Prdx-1, we observed changes in reactive oxygen species (ROS) in naringenin-treated SNU-213 cells. The ROS levels were 130.02 +/- 20.21%, 182.04 +/- 5.39%, and 237.21 +/- 12.71% in response to 200, 400, and 600 mu M naringenin treatment, respectively. Increases in ROS were followed by up-regulation of apoptosis signal-regulation kinase 1 (ASK1). Moreover, the JNK, p38 and p53 proteins were upregulated. Overall, the results of this study suggest that naringenin causes ASK1-induced apoptosis mediated by ROS. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.subject | ANTIOXIDANT | - |
dc.subject | MECHANISMS | - |
dc.subject | FLAVONOIDS | - |
dc.subject | PROLIFERATION | - |
dc.subject | INHIBITION | - |
dc.subject | STATISTICS | - |
dc.subject | ACTIVATION | - |
dc.subject | SURVIVAL | - |
dc.title | Naringenin causes ASK1-induced apoptosis via reactive oxygen species in human pancreatic cancer cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000392789800001 | - |
dc.identifier.doi | 10.1016/j.fct.2016.11.008 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.99, pp.1 - 8 | - |
dc.identifier.scopusid | 2-s2.0-84995380655 | - |
dc.citation.endPage | 8 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 99 | - |
dc.contributor.affiliatedAuthor | Park, Hyun Jin | - |
dc.contributor.affiliatedAuthor | Choi, Yong Jun | - |
dc.contributor.affiliatedAuthor | Lee, Jeong Hyun | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Naringenin | - |
dc.subject.keywordAuthor | Pancreatic cancer | - |
dc.subject.keywordAuthor | Peroxiredoxin-1 | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Apoptosis signal-regulation kinase 1 | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | FLAVONOIDS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | STATISTICS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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