Design, Synthesis, Structure-Activity Relationship and Docking Studies of Novel Functionalized Arylvinyl-1,2,4-Trioxanes as Potent Antiplasmodial as well as Anticancer Agents
- Authors
- Tiwari M.K.; Coghi P.; Agrawal P.; Shyamlal B.R.K.; Jun Yang L.; Yadav L.; Peng Y.; Sharma R.; Yadav D.K.; Sahal D.; Kam Wai Wong V.; Chaudhary S.
- Issue Date
- Jul-2020
- Publisher
- John Wiley and Sons Ltd
- Keywords
- anticancer; antiplasmodial; artemisinin; artesunate; trioxane
- Citation
- ChemMedChem, v.15, no.13, pp.1216 - 1228
- Journal Title
- ChemMedChem
- Volume
- 15
- Number
- 13
- Start Page
- 1216
- End Page
- 1228
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/67592
- DOI
- 10.1002/cmdc.202000045
- ISSN
- 1860-7179
- Abstract
- A novel series of synthetic functionalized arylvinyl-1,2,4-trioxanes (8 a–p) has been prepared and assessed for their in vitro antiplasmodial activity against the chloroquine-resistant Pf INDO strain of Plasmodium falciparum by using a SYBR green-I fluorescence assay. Compounds 8 g (IC50=0.051 μM; SI=589.41) and 8 m (IC50=0.059 μM; SI=55.93) showed 11-fold and >9-fold more potent antiplasmodial activity, respectively, as compared to chloroquine (IC50=0.546 μM; SI=36.63). Different in silico docking studies performed on many target proteins revealed that the most active arylvinyl-1,2,4-trioxanes (8 g and 8 m) showed dihydrofolate reductase (DHFR) binding affinities on a par with those of chloroquine and artesunate. The in vitro cytotoxic potentials of 8 a–p were also evaluated against human lung (A549) and liver (HepG2) cancer cell lines along with immortalized normal lung (BEAS-2B) and liver (LO2) cell lines. Following screening, five derivatives viz. 8 a, 8 h, 8 l, 8 m and 8 o (IC50=1.65–31.7 μM; SI=1.08–10.96) were found to show potent cytotoxic activity against (A549) lung cancer cell lines, with selectivity superior to that of the reference compounds artemisinin (IC50=100 μM), chloroquine (IC50=100 μM) and artesunic acid (IC50=9.85 μM; SI=0.76). In fact, the most active 4-naphthyl-substituted analogue 8 l (IC50=1.65 μM; SI >10) exhibited >60 times more cytotoxicity than the standard reference, artemisinin, against A549 lung cancer cell lines. In silico docking studies of the most active anticancer compounds, 8 l and 8 m, against EGFR were found to validate the wet lab results. In summary, a new series of functionalized aryl-vinyl-1,2,4-trioxanes (8 a–p) has been shown to display dual potency as promising antiplasmodial and anticancer agents. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
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