금속이온, 킬레이트 화합물과 키토산 혼합물의 항칸디다 상승 효과

Abstract

The potential of using chitosan as a biological agent for the control of Candida albicans was investigated. A chitosan designated as an active molecular chitosan (AMC) with average molecular weight of 8.5-kDa exhibited an effective and potential anticandidal effect at 100 mg/mL. Although metal ion Zn 2+ and EDTA had shown the significant growth inhibitory activity, but both had also shown the static effect on reversible growth inhibition, respectively, whereas AMC showed irreversible growth inhibition. In addition, anticandidal activity of those metal ions, chelating agent and a classic drug using as anticandidal agent Amphotericin B was greatly increased in the presence of AMC mg/mL. These results suggest that AMC-Zn 2+ - EDTA exerted their activities through dysregulation of the homeostasis or signaling of different cations in anticandidal effect. Thus, the combination of Zn 2+ , chelating agent EDTA and AMC may be an effective disease management strategy to prevent or delay the development of bactericidal-resistant strains.