Analysis of the Apoptotic Mechanisms of Snake Venom Toxin on Inflammation-induced HaCaT Cell-line
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전열웅 | - |
dc.contributor.author | 송호섭 | - |
dc.date.available | 2020-02-27T22:43:55Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 2586-288X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7414 | - |
dc.description.abstract | Objectives : In this study, the roles of Interleukin (IL)-4 and Signal transducer and activator of transcription 6 (STAT6), which have been reported to play a role in the pathogenesis of inflammation and cancer, were evaluated in snake venom toxin (SVT)-induced apoptosis. Methods : Inflammation was induced in human HaCaT kerationocytes, by lipopolysaccharide (LPS; 1 μg/mL) or tumor necrosis factor-α (TNF-α), followed by treatment with SVT (0, 1, or 2 μg/mL). Cell viability was assessed by MTT assays after 24 h, and the expression of levels of IL-4, STAT6, and the apoptosis-related proteins p53, Bax, and Bcl-2 were evaluated by western blotting. Electro mobility shift assays (EMSAs) were performed to evaluate the DNA binding capacity of STAT6. Results : MTT assays showed that inflammation-induced growth of HaCaT cells following LPS or TNF-α stimulation was inhibited by SVT. Western blot analysis showed that p53 and Bax, which promote apoptosis, were increased, whereas that of Bcl-2, an anti-apoptotic protein, was decreased in a concentration-dependent manner in LPS- or TNF-α-induced HaCaT cells following treatment with SVT. Moreover, following treatment of HaCaT cells with LPS, IL-4 concentrations were increased, and treatment with SVT further increased IL-4 expression in a concentration-dependent manner. Western blotting and EMSAs showed that the phosphorylated form of STAT6 was increased in HaCaT cells in the context of LPS- or TNF-α-induced inflammation in a concentration-dependent manner, concomitant with an increase in the DNA binding activity of STAT6. Conclusion : SVT can effectively promote apoptosis in HaCaT cells in the presence of inflammation through a pathway involving IL-4 and STAT6 | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 대한침구의학회 | - |
dc.relation.isPartOf | Journal of Acupuncture Research | - |
dc.title | Analysis of the Apoptotic Mechanisms of Snake Venom Toxin on Inflammation-induced HaCaT Cell-line | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.bibliographicCitation | Journal of Acupuncture Research, v.34, no.1, pp.23 - 30 | - |
dc.identifier.kciid | ART002196328 | - |
dc.citation.endPage | 30 | - |
dc.citation.startPage | 23 | - |
dc.citation.title | Journal of Acupuncture Research | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 전열웅 | - |
dc.contributor.affiliatedAuthor | 송호섭 | - |
dc.subject.keywordAuthor | Snake venom toxin | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Interleukin-4 | - |
dc.subject.keywordAuthor | Signal transducer and activator of transcription 6 | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.