Identification and Characterization of a Splicing Variant in the 5 ' UTR of the Human TLR5 Gene
DC Field | Value | Language |
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dc.contributor.author | Hoang, Thi Xoan | - |
dc.contributor.author | Duong, Cao Nguyen | - |
dc.contributor.author | Kim, Jae Young | - |
dc.date.available | 2020-02-27T23:41:46Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7485 | - |
dc.description.abstract | Toll-like receptors (TLRs) are essential components of the innate immune system. TLR5 is the receptor for flagellin, the principal protein component of bacterial flagella. The TLR5 gene has 6 exons. In an RT-PCR analysis, we found long TLR5 transcripts, in addition to those of the expected size (short TLR5 transcripts). A sequence analysis revealed that the long TLR5 transcripts contain a new exon of 94 nucleotides located between previously reported exons IV and V in the 5' untranslated region (5.. UTR). A realtime PCR analysis of the two alternatively spliced variants in various cell lines showed that the long TLR5 transcripts are abundantly expressed in nonimmune cells. The ratios of long/short transcripts in human nonimmune cell lines, such as A549, T98G, HaCaT, H460, HEK-293, and Caco-2 cells, and primary mesenchymal stemcells were in the range of 1.25 to 4.31. In contrast, those of human monocytic THP-1 and U937 cells and E6.1 T cells and Ramos B cells were around 0.9. These ratios in humanmonocytic THP-1 cells were decreased by treatment with IFN-gamma in a concentration-dependent manner. Based on our findings, we suggest that the newly found long TLR5 transcripts may be involved in the negative regulation of TLR5 expression and function. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | HINDAWI LTD | - |
dc.relation.isPartOf | BIOMED RESEARCH INTERNATIONAL | - |
dc.title | Identification and Characterization of a Splicing Variant in the 5 ' UTR of the Human TLR5 Gene | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000408618600001 | - |
dc.identifier.doi | 10.1155/2017/8727434 | - |
dc.identifier.bibliographicCitation | BIOMED RESEARCH INTERNATIONAL | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85029813971 | - |
dc.citation.title | BIOMED RESEARCH INTERNATIONAL | - |
dc.contributor.affiliatedAuthor | Hoang, Thi Xoan | - |
dc.contributor.affiliatedAuthor | Duong, Cao Nguyen | - |
dc.contributor.affiliatedAuthor | Kim, Jae Young | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | TOLL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | MYD88 | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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