Photodynamic therapy by conjugation of cell-penetrating peptide with fluorochrome
DC Field | Value | Language |
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dc.contributor.author | Park, Chul-Kyu | - |
dc.contributor.author | Kim, Yong Ho | - |
dc.contributor.author | Hwangbo, Suhyun | - |
dc.contributor.author | Cho, Hoonsung | - |
dc.date.available | 2020-02-27T23:42:27Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1178-2013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7535 | - |
dc.description.abstract | Photodynamic therapy (PDT) is a promising alternative therapy that could be used as an adjunct to chemotherapy and surgery for cancer, and works by destroying tissue with visible light in the presence of a photosensitizer (PS) and oxygen. The PS should restrict tissue destruction only to the tumor and be activated by light of a specific wavelength; both of these properties are required. Arginine-rich peptides, such as cell-penetrating peptides, have membrane-translocating and nuclear-localizing activities, which have led to their application in various drug delivery modalities. Protamine (Pro) is an arginine-rich peptide with membrane-translocating and nuclear-localizing properties. The reaction of an N-hydroxysuccinimide (NHS) ester of rhodamine (Rho) and clinical Pro was carried out in this study to yield RhoPro, and a demonstration of its phototoxicity, wherein clinical Pro improved the effect of PDT, was performed. The reaction between Pro and the NHS ester of Rho is a solution-phase reaction that results in the complete modification of the Pro peptides, which feature a single reactive amine at the N-terminal proline and a single carboxyl group at the C-terminal arginine. This study aimed to identify a new type of PS for PDT by in vitro and in vivo experiments and to assess the antitumor effects of PDT, using the Pro-conjugated PS, on a cancer cell line. Photodynamic cell death studies showed that the RhoPro produced has more efficient photodynamic activities than Rho alone, causing rapid light-induced cell death. The attachment of clinical Pro to Rho, yielding RhoPro, confers the membrane-internalizing activity of its arginine-rich content on the fluorochrome Rho and can induce rapid photodynamic cell death, presumably owing to light-induced cell membrane rupture. PDT using RhoPro for HT-29 cells was very effective and these findings suggest that RhoPro is a suitable candidate as a PS for solid tumors. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | DOVE MEDICAL PRESS LTD | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.subject | LYSOSOMAL MEMBRANE PERMEABILIZATION | - |
dc.subject | DEATH | - |
dc.subject | PATHWAYS | - |
dc.subject | DELIVERY | - |
dc.subject | CANCER | - |
dc.title | Photodynamic therapy by conjugation of cell-penetrating peptide with fluorochrome | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000414864900001 | - |
dc.identifier.doi | 10.2147/IJN.S148332 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF NANOMEDICINE, v.12, pp.8185 - 8196 | - |
dc.identifier.scopusid | 2-s2.0-85034456014 | - |
dc.citation.endPage | 8196 | - |
dc.citation.startPage | 8185 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.citation.volume | 12 | - |
dc.contributor.affiliatedAuthor | Park, Chul-Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Yong Ho | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | photodynamic therapy | - |
dc.subject.keywordAuthor | rhodamine | - |
dc.subject.keywordAuthor | protamine | - |
dc.subject.keywordAuthor | endocytosis | - |
dc.subject.keywordAuthor | cell-penetrating peptide | - |
dc.subject.keywordPlus | LYSOSOMAL MEMBRANE PERMEABILIZATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | CANCER | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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