Phospholipase D1 deficiency in mice causes nonalcoholic fatty liver disease via an autophagy defect
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hur, Jang Ho | - |
dc.contributor.author | Park, Shi-Young | - |
dc.contributor.author | Dall'Armi, Claudia | - |
dc.contributor.author | Lee, Jae Sung | - |
dc.contributor.author | Di Paolo, Gilbert | - |
dc.contributor.author | Lee, Hui-Young | - |
dc.contributor.author | Yoon, Mee-Sup | - |
dc.contributor.author | Min, Do Sik | - |
dc.contributor.author | Choi, Cheol Soo | - |
dc.date.available | 2020-02-27T23:43:14Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-12-15 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7586 | - |
dc.description.abstract | Nonalcoholic fatty liver disease (NAFLD) is characterized by the accumulation of triglycerides (TG) as lipid droplets in the liver. Although lipid-metabolizing enzymes are considered important in NAFLD, the involvement of phospholipase D1 (PLD1) has not yet been studied. Here, we show that the genetic ablation of PLD1 in mice induces NAFLD due to an autophagy defect. PLD1 expression was decreased in high-fat diet-induced NAFLD. Subsequently, PLD1 deficiency led to an increase in hepatic TGs and liver weight. Autophagic flux was blocked in Pld1(-/-) hepatocytes, with decreased beta-oxidation rate, reduced oxidation-related gene expression, and swollen mitochondria. The dynamics of autophagy was restored by treatment with the PLD product, phosphatidic acid (PA) or adenoviral PLD1 expression in Pld1(-/-) hepatocytes, confirming that lysosomal PA produced by PLD1 regulates autophagy. Notably, PLD1 expression in Pld1(-/-) liver significantly reduced hepatic lipid accumulation, compared with Pld1(-/-) liver. Thus, PLD1 plays an important role in hepatic steatosis via the regulation of autophagy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.subject | HEPATIC INSULIN-RESISTANCE | - |
dc.subject | PHOSPHATIDIC-ACID | - |
dc.subject | DIACYLGLYCEROL | - |
dc.subject | CELLS | - |
dc.subject | MTOR | - |
dc.subject | SENSITIVITY | - |
dc.subject | METABOLISM | - |
dc.subject | STEATOSIS | - |
dc.subject | HEPATOCYTES | - |
dc.subject | ACTIVATION | - |
dc.title | Phospholipase D1 deficiency in mice causes nonalcoholic fatty liver disease via an autophagy defect | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000392092600001 | - |
dc.identifier.doi | 10.1038/srep39170 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.identifier.scopusid | 2-s2.0-85006284712 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Hur, Jang Ho | - |
dc.contributor.affiliatedAuthor | Park, Shi-Young | - |
dc.contributor.affiliatedAuthor | Lee, Jae Sung | - |
dc.contributor.affiliatedAuthor | Lee, Hui-Young | - |
dc.contributor.affiliatedAuthor | Yoon, Mee-Sup | - |
dc.contributor.affiliatedAuthor | Choi, Cheol Soo | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HEPATIC INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | DIACYLGLYCEROL | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MTOR | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | STEATOSIS | - |
dc.subject.keywordPlus | HEPATOCYTES | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.