Anti-apoptotic and antioxidant effects of 3-epi-iso- seco-tanapartholide isolated from artemisia argyi against iodixanol-induced kidney epithelial cell death
DC Field | Value | Language |
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dc.contributor.author | Lee D. | - |
dc.contributor.author | Kim K.O. | - |
dc.contributor.author | Lee D. | - |
dc.contributor.author | Kang K.S. | - |
dc.date.available | 2020-08-10T00:36:34Z | - |
dc.date.created | 2020-06-17 | - |
dc.date.issued | 2020-06 | - |
dc.identifier.issn | 2218-273X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/76192 | - |
dc.description.abstract | Iodixanol is a non-ionic iso-osmolar contrast agent, but it is a risk factor for kidney damage and increases morbidity and mortality. In this study, we investigated the effect of 9 sesquiterpenes isolated from mugwort (Artemisia argyi) in contrast agent-induced cytotoxicity in LLC-PK1 cells. Cells were exposed to nine sesquiterpene compounds for 2 h, followed by incubation with iodixanol for 3 h. Cell viability was assessed using the Ez-Cytox assay. The level of reactive oxygen species was measured using 2′,7′-dichlorodihydrofluorescein diacetate staining. Apoptotic cell death was detected using annexin V/PI staining. In addition, immunofluorescence staining and western blotting were performed using antibodies against proteins related to apoptosis, oxidative stress, and MAPK pathways. The most effective 3-epi-iso-seco-tanapartholide (compound 8) among the 9 sesquiterpene compounds protected LLC-PK1 cells from iodixanol-induced cytotoxicity, oxidative stress, and apoptotic cell death. Pretreatment with compound 8 reversed iodixanol-induced increases in the expression of JNK, ERK, p38, Bax, caspase-3, and caspase-9. It also reversed the iodixanol-induced decrease in Bcl-2 expression. Furthermore, pretreatment with compound 8 caused nuclear translocation of Nrf2 and upregulated HO-1 via the Nrf2 pathway in iodixanol-treated LLC-PK1 cells. Thus, we demonstrated here that compound 8 isolated from A. argyi has the potential to effectively prevent iodixanol-induced kidney epithelial cell death via the caspase- 3/MAPK pathways and HO-1 via the Nrf2 pathway. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI AG | - |
dc.relation.isPartOf | Biomolecules | - |
dc.title | Anti-apoptotic and antioxidant effects of 3-epi-iso- seco-tanapartholide isolated from artemisia argyi against iodixanol-induced kidney epithelial cell death | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000550863800001 | - |
dc.identifier.doi | 10.3390/biom10060867 | - |
dc.identifier.bibliographicCitation | Biomolecules, v.10, no.6, pp.1 - 18 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85086176083 | - |
dc.citation.endPage | 18 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Biomolecules | - |
dc.citation.volume | 10 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Lee D. | - |
dc.contributor.affiliatedAuthor | Kang K.S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Contrast agent | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Iodixanol | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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