An integrated microfluidic PCR system with immunomagnetic nanoparticles for the detection of bacterial pathogens
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ganesh, Irisappan | - |
dc.contributor.author | Buu Minh Tran | - |
dc.contributor.author | Kim, Yonghee | - |
dc.contributor.author | Kim, Jaewon | - |
dc.contributor.author | Cheng, Hua | - |
dc.contributor.author | Lee, Nae Yoon | - |
dc.contributor.author | Park, Sungsu | - |
dc.date.available | 2020-02-27T23:44:02Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 1387-2176 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7645 | - |
dc.description.abstract | There is growing interest in rapid microbial preconcentration methods to lower the detection limit of bacterial pathogens of low abundance in samples. Here, we report an integrated microfluidic PCR system that enables bacterial cells of interest in samples to be concentrated prior to PCR. It consists of two major compartments: a preconcentration chamber for the immunomagnetic separation of bacterial cells, and a PCR chamber for the DNA amplification of the concentrated cells. We demonstrate the feasibility of the system for the detection of microbial pathogens by preconcentrating the human pathogen Escherichia coli O157: H7, and also amplifying its DNA. The detection limit of E. coli O157: H7 in the PCR system is 1 x 103 CFU (colony forming unit)/mL. Onchip processing steps, including preconcentration and PCR steps, take less than two hours. Our system can serve as a rapid, specific, and quantitative platform for the detection of microbial pathogens in samples of large volume. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.relation.isPartOf | BIOMEDICAL MICRODEVICES | - |
dc.subject | ESCHERICHIA-COLI O157-H7 | - |
dc.subject | REAL-TIME PCR | - |
dc.subject | SAMPLE PREPARATION | - |
dc.subject | IMPEDANCE BIOSENSOR | - |
dc.subject | DNA EXTRACTION | - |
dc.subject | LABEL-FREE | - |
dc.subject | SEPARATION | - |
dc.subject | CELLS | - |
dc.subject | O157/H7 | - |
dc.subject | PROTEIN | - |
dc.title | An integrated microfluidic PCR system with immunomagnetic nanoparticles for the detection of bacterial pathogens | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000391424600020 | - |
dc.identifier.doi | 10.1007/s10544-016-0139-y | - |
dc.identifier.bibliographicCitation | BIOMEDICAL MICRODEVICES, v.18, no.6 | - |
dc.identifier.scopusid | 2-s2.0-85003782012 | - |
dc.citation.title | BIOMEDICAL MICRODEVICES | - |
dc.citation.volume | 18 | - |
dc.citation.number | 6 | - |
dc.contributor.affiliatedAuthor | Buu Minh Tran | - |
dc.contributor.affiliatedAuthor | Lee, Nae Yoon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Escherichia coli O157: H7 | - |
dc.subject.keywordAuthor | Microfluidic device | - |
dc.subject.keywordAuthor | Immunomagnetic separation | - |
dc.subject.keywordAuthor | On-chip PCR | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI O157-H7 | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | SAMPLE PREPARATION | - |
dc.subject.keywordPlus | IMPEDANCE BIOSENSOR | - |
dc.subject.keywordPlus | DNA EXTRACTION | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | O157/H7 | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.