Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, HyeonSeok | - |
dc.contributor.author | Hong, Seong-Joo | - |
dc.contributor.author | Yoo, Chan | - |
dc.contributor.author | Han, Mi-Ae | - |
dc.contributor.author | Lee, Hookeun | - |
dc.contributor.author | Choi, Hyung-Kyoon | - |
dc.contributor.author | Cho, Suhyung | - |
dc.contributor.author | Lee, Choul-Gyun | - |
dc.contributor.author | Cho, Byung-Kwan | - |
dc.date.available | 2020-02-27T23:44:29Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-11-24 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7681 | - |
dc.description.abstract | Temperature is a critical environmental factor that affects microalgal growth. However, microalgal coping mechanisms for temperature variations are unclear. Here, we determined changes in transcriptome, total carbohydrate, total fatty acid methyl ester, and fatty acid composition of Tetraselmis sp. KCTC12432BP, a strain with a broad temperature tolerance range, to elucidate the tolerance mechanisms in response to large temperature variations. Owing to unavailability of genome sequence information, de novo transcriptome assembly coupled with BLAST analysis was performed using strand specific RNA-seq data. This resulted in 26,245 protein-coding transcripts, of which 83.7% could be annotated to putative functions. We identified more than 681 genes differentially expressed, suggesting an organelle-specific response to temperature variation. Among these, the genes related to the photosynthetic electron transfer chain, which are localized in the plastid thylakoid membrane, were upregulated at low temperature. However, the transcripts related to the electron transport chain and biosynthesis of phosphatidylethanolamine localized in mitochondria were upregulated at high temperature. These results show that the low energy uptake by repressed photosynthesis under low and high temperature conditions is compensated by different mechanisms, including photosystem I and mitochondrial oxidative phosphorylation, respectively. This study illustrates that microalgae tolerate different temperature conditions through organelle specific mechanisms. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.subject | RNA-SEQ DATA | - |
dc.subject | PHOTOSYSTEM-II | - |
dc.subject | HEAT-STRESS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | PHOTOSYNTHESIS | - |
dc.subject | MICROALGAE | - |
dc.subject | PHOSPHATIDYLETHANOLAMINE | - |
dc.subject | PATHWAY | - |
dc.subject | GROWTH | - |
dc.subject | PHOSPHATIDYLGLYCEROL | - |
dc.title | Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000392067900002 | - |
dc.identifier.doi | 10.1038/srep37770 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.identifier.scopusid | 2-s2.0-84997427353 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Lee, Hookeun | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RNA-SEQ DATA | - |
dc.subject.keywordPlus | PHOTOSYSTEM-II | - |
dc.subject.keywordPlus | HEAT-STRESS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PHOTOSYNTHESIS | - |
dc.subject.keywordPlus | MICROALGAE | - |
dc.subject.keywordPlus | PHOSPHATIDYLETHANOLAMINE | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | PHOSPHATIDYLGLYCEROL | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.