PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639
DC Field | Value | Language |
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dc.contributor.author | Song, Hae-In | - |
dc.contributor.author | Yoon, Mee-Sup | - |
dc.date.available | 2020-02-27T23:44:30Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-11-22 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7682 | - |
dc.description.abstract | Phospholipase D1 (PLD1) plays a known role in several differentiation processes, but its role in adipogenic differentiation remains unknown. In the present study, we identified PLD1 as a negative regulator of adipogenic differentiation. We showed that PLD activity was downregulated by both 3-Isobutyl-1-methylxanthine (IBMX) and insulin upon induction of differentiation in 3T3-L1 adipogenic cells. In line with this observation, PLD activity decreased in both high fat diet (HFD)-fed mice and ob/ob mice. We also found that differentiation of 3T3-L1 preadipocytes was enhanced by the depletion of PLD1 levels or inhibition of PLD1 activity by VU0155069, a PLD1-specific inhibitor. Conversely, treatment with phosphatidic acid (PA), a PLD product, and overexpression of PLD1 both caused a decrease in adipogenic differentiation. Moreover, the elevated differentiation in PLD1-knockdown 3T3-L1 cells was reduced by either PA treatment or PLD1 expression, confirming negative roles of PLD1 and PA in adipogenic differentiation. Further investigation revealed that PA displaces DEP domain-containing mTOR-interacting protein (DEPTOR) from mTORC1, which subsequently phosphorylates insulin receptor substrate-1 (IRS-1) at serine 636/639 in 3T3-L1 cells. Taken together, our findings provide convincing evidence for a direct role of PLD1 in adipogenic differentiation by regulating IRS-1 phosphorylation at serine 636/639 through DEPTOR displacement and mTOR activation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.subject | CYCLIC PHOSPHATIDIC-ACID | - |
dc.subject | CELL-FREE SYSTEM | - |
dc.subject | COMPLEX 1 MTORC1 | - |
dc.subject | PHOSPHOLIPASE-D | - |
dc.subject | PPAR-GAMMA | - |
dc.subject | ADIPOCYTE DIFFERENTIATION | - |
dc.subject | MAMMALIAN TARGET | - |
dc.subject | ABDOMINAL ADIPOSITY | - |
dc.subject | HUMAN NEUTROPHILS | - |
dc.subject | BINDING-PROTEIN | - |
dc.title | PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000388223700001 | - |
dc.identifier.doi | 10.1038/srep36968 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6 | - |
dc.identifier.scopusid | 2-s2.0-84996598187 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.affiliatedAuthor | Song, Hae-In | - |
dc.contributor.affiliatedAuthor | Yoon, Mee-Sup | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CYCLIC PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | CELL-FREE SYSTEM | - |
dc.subject.keywordPlus | COMPLEX 1 MTORC1 | - |
dc.subject.keywordPlus | PHOSPHOLIPASE-D | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | MAMMALIAN TARGET | - |
dc.subject.keywordPlus | ABDOMINAL ADIPOSITY | - |
dc.subject.keywordPlus | HUMAN NEUTROPHILS | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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