Development of Interdigitated and Chain-Shaped Electrode Array for Electric Cell-Substrate Impedance Sensing

Abstract

Interdigitated and chain-shaped electrode (ICE) array was developed for improving the electric cellsubstrate impedance sensing. A newly designed ICE with a sensing area of 0.12 to 0.88 mm(2), and finger width and spacing of 20 mu m was fabricated. An equivalent circuit model was used to characterize the measured impedance spectra, and the circuit parameters extrapolated by fitting in the equivalent circuit model of the measured spectra were analyzed during the cell cultivation on the ICE. During the cell growth, the real part of the impedance was increased in the frequency range of 100 Hz to 220 kHz. The resistance measurement sensitivity of the cells on the ICE was the highest at a frequency of 46.4 kHz, and it increased with decrease in the electrode sensing area.