Mutant DNA Detection on Graphene Oxide with SYBR Green I
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Chunghyun | - |
dc.contributor.author | Gang, Jongback | - |
dc.date.available | 2020-02-28T00:41:22Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7738 | - |
dc.description.abstract | Graphene oxide (GO) has a large surface-to-volume ratio and contains hydrophobic hexagonal rings that can interact with biomolecules. Single-stranded DNA (ssDNA) adsorbs more strongly to the surface of GO than double stranded DNA (dsDNA) via hydrophobic interaction between nucleobases of DNA and the hydrophobic surface of GO. This study was designed to detect samples containing various mismatched DNAs under different pH and salt conditions by sequential use of GO and SYBR Green I. Our results demonstrated that a GO-based fluorescence assay showed little difference in fluorescence intensity between perfectly matched DNA (pmDNA) and mutant DNAs. However, an SYBR Green I-assisted assay elicited large difference in fluorescence intensity between the pmDNA and mismatched DNAs. This enhances the detection sensitivity and efficiency between pmDNA and mutant DNA. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.relation.isPartOf | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.title | Mutant DNA Detection on Graphene Oxide with SYBR Green I | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000387278200145 | - |
dc.identifier.doi | 10.1166/jnn.2016.13609 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.16, no.11, pp.11868 - 11872 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84992491265 | - |
dc.citation.endPage | 11872 | - |
dc.citation.startPage | 11868 | - |
dc.citation.title | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.citation.volume | 16 | - |
dc.citation.number | 11 | - |
dc.contributor.affiliatedAuthor | Lee, Chunghyun | - |
dc.contributor.affiliatedAuthor | Gang, Jongback | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Graphene Oxide | - |
dc.subject.keywordAuthor | DNA Adsorption | - |
dc.subject.keywordAuthor | Mutation Detection | - |
dc.subject.keywordAuthor | Reaction Condition | - |
dc.subject.keywordAuthor | Mismatched DNA | - |
dc.subject.keywordAuthor | SYBR Green I | - |
dc.subject.keywordPlus | MUTATION DETECTION | - |
dc.subject.keywordPlus | GRADIENT ELECTROPHORESIS | - |
dc.subject.keywordPlus | STRANDED-DNA | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.