Genistein induces apoptosis by down-regulating thioredoxin-1 in human hepatocellular carcinoma SNU-449 cells
DC Field | Value | Language |
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dc.contributor.author | Roh, Taylor | - |
dc.contributor.author | Kim, Sung Won | - |
dc.contributor.author | Moon, Soung Hoon | - |
dc.contributor.author | Nam, Myeong Jin | - |
dc.date.available | 2020-02-28T00:41:23Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.issn | 0278-6915 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/7739 | - |
dc.description.abstract | Genistein (GEN), a natural isoflavonoid phytoestrogen, has anti-cancer activity against various types of cancers. However, GEN has not been thoroughly investigated in human hepatocellular carcinoma cells. In this study, we evaluated the anti-cancer effects of GEN on SNU-449 cells. GEN inhibited the proliferation of SNU-449 cells in a concentration-dependent manner. We observed the typical characteristics of apoptosis, such as DNA fragmentation and caspase-3 activation. To identify proteins related to GEN-induced apoptosis, we performed two-dimensional electrophoresis and identified differentially expressed proteins. Proteomic analysis showed that the antioxidant protein thioredoxin-1 was associated with GEN-induced apoptosis. GEN treatment decreased thioredoxin-1 levels and increased intracellular accumulation of reactive oxygen species. In addition, GEN activated apoptosis signal-regulating kinase 1, c-Jun N-terminal kinases (JNK) and p38. We also observed that pretreatment with the JNK and p38 inhibitors (SP600125 and SB203580) decreased GEN-induced cell death. These results indicate that GEN has potential antitumor effects against SNU-449 cells through the down-regulation of thioredoxin-1. (C) 2016 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.relation.isPartOf | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.subject | COLON-CANCER CELLS | - |
dc.subject | MAMMALIAN THIOREDOXIN | - |
dc.subject | BIOMARKER DISCOVERY | - |
dc.subject | PROSTATE-CANCER | - |
dc.subject | BREAST-CANCER | - |
dc.subject | EXPRESSION | - |
dc.subject | PROTEINS | - |
dc.subject | GROWTH | - |
dc.subject | INHIBITORS | - |
dc.subject | PROTEOMICS | - |
dc.title | Genistein induces apoptosis by down-regulating thioredoxin-1 in human hepatocellular carcinoma SNU-449 cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000387627000013 | - |
dc.identifier.doi | 10.1016/j.fct.2016.09.003 | - |
dc.identifier.bibliographicCitation | FOOD AND CHEMICAL TOXICOLOGY, v.97, pp.127 - 134 | - |
dc.identifier.scopusid | 2-s2.0-84986550425 | - |
dc.citation.endPage | 134 | - |
dc.citation.startPage | 127 | - |
dc.citation.title | FOOD AND CHEMICAL TOXICOLOGY | - |
dc.citation.volume | 97 | - |
dc.contributor.affiliatedAuthor | Roh, Taylor | - |
dc.contributor.affiliatedAuthor | Kim, Sung Won | - |
dc.contributor.affiliatedAuthor | Moon, Soung Hoon | - |
dc.contributor.affiliatedAuthor | Nam, Myeong Jin | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Genistein | - |
dc.subject.keywordAuthor | Hepatocellular carcinoma | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Thioredoxin-1 | - |
dc.subject.keywordPlus | COLON-CANCER CELLS | - |
dc.subject.keywordPlus | MAMMALIAN THIOREDOXIN | - |
dc.subject.keywordPlus | BIOMARKER DISCOVERY | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | BREAST-CANCER | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | PROTEOMICS | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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