An innovative blood plasma separation method for a paper-based analytical device using chitosan functionalization
- Authors
- Kim, Dami; Kim, Sejin; Kim, Sanghyo
- Issue Date
- Aug-2020
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ANALYST, v.145, no.16, pp.5491 - 5499
- Journal Title
- ANALYST
- Volume
- 145
- Number
- 16
- Start Page
- 5491
- End Page
- 5499
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78036
- DOI
- 10.1039/d0an00500b
- ISSN
- 0003-2654
- Abstract
- This study describes a microfluidic paper-based analytical device (μPAD) for separating plasma from whole blood and measuring glucose concentration. A two-dimensional μPAD was fabricated by wax printing, using chromatographic paper and was functionalized by the incorporation of chitosan into the plasma separation zone. Red blood cells were isolated from whole blood in the plasma separation zone consisting of a chitosan polymer structure and a wax barrier. The separated plasma, with buffer, was injected into the test zone to cause a color change. The colorimetric results were digitized with a scanner, and the concentration was calculated using a calibration curve. This μPAD, which separated the plasma from whole blood without a separation membrane, will be useful for constructing point-of-care testing devices that detect analytes in small sample volumes.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78036)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.