Microneedle array sensor for monitoring glucose in single cell using glucose oxidase-bonded polyterthiophene coated on AuZn oxide layer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee W.-C. | - |
dc.contributor.author | Gurudatt N.G. | - |
dc.contributor.author | Park D.-S. | - |
dc.contributor.author | Kim K.B. | - |
dc.contributor.author | Choi C.S. | - |
dc.contributor.author | Shim Y.-B. | - |
dc.date.available | 2020-08-28T02:35:21Z | - |
dc.date.created | 2020-06-15 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78060 | - |
dc.description.abstract | A monomer precursor of conducting polymer, 2,2´:5´,5″-terthiophene-3´-carboxylic acid, (TCA) was electropolymerized on the raspberry-like AuZn oxide (AuZnOx) layer coated on microneedle array electrode (μ-NAE) at single and multi-cell analysis. The AuZnOx was used to elevate the catalytic effect of H2O2 oxidation and to prevent the passivation of the Au surface by chloride ion in biological samples. After optimizing the experimental variables affecting the analytical signal, in terms of monomer and GOx concentrations, electropolymerization cycles, pH, applied potential, and temperature. The sensor was responded with in 1 s, and showed the two dynamic ranges of 0.01–5.0 and 5.0–50.0 mM with detection limit of 1.2 (± 0.026) μM. The sensor performance and reliability were examined by measuring glucose in cells of different cancer types (PC-12, MG-62, HeLa, A549, and Saos-2), comparing with normal (Vero) cells. In addition, the effect of K+ ion on the glucose level was also investigated for a single cell. The sensor confirmed the higher concentration of glucose in cancer cells than the normal cells, where the intracellular glucose level was 2.2 folds higher as compared to the extracellular levels in PC-12 cells. © 2020 Elsevier B.V. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier B.V. | - |
dc.relation.isPartOf | Sensors and Actuators, B: Chemical | - |
dc.title | Microneedle array sensor for monitoring glucose in single cell using glucose oxidase-bonded polyterthiophene coated on AuZn oxide layer | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000558996400006 | - |
dc.identifier.doi | 10.1016/j.snb.2020.128416 | - |
dc.identifier.bibliographicCitation | Sensors and Actuators, B: Chemical, v.320 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85086100310 | - |
dc.citation.title | Sensors and Actuators, B: Chemical | - |
dc.citation.volume | 320 | - |
dc.contributor.affiliatedAuthor | Choi C.S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Array | - |
dc.subject.keywordAuthor | AuZnOx | - |
dc.subject.keywordAuthor | Cell analysis | - |
dc.subject.keywordAuthor | Conducting polymer | - |
dc.subject.keywordAuthor | Glucose monitoring | - |
dc.subject.keywordAuthor | Microneedle | - |
dc.subject.keywordAuthor | Raspberry-like | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.