Cell-specific expression of ENACα gene by FOXA1 in the glucocorticoid receptor pathway
DC Field | Value | Language |
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dc.contributor.author | Chung, Y.S. | - |
dc.contributor.author | Jin, H.L. | - |
dc.contributor.author | Jeong, K.W. | - |
dc.date.available | 2020-09-16T01:35:37Z | - |
dc.date.created | 2020-09-03 | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 0394-6320 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78222 | - |
dc.description.abstract | INTRODUCTION: The glucocorticoid receptor (GR) is one of the most widely studied ligand-dependent nuclear receptors. The combination of transcriptional regulatory factors required for the expression of individual genes targeted by GR varies across cell types; however, the mechanisms underlying this cell type-specific regulation of gene expression are not yet clear. METHODS: Here, we investigated genes regulated by GR in two different cell lines, A549 and ARPE-19, and examined how gene expression varied according to the effect of pioneer factors using RNA-seq and RT-qPCR. RESULTS: Our RNA-seq results identified 19 and 63 genes regulated by GR that are ARPE-19-specific and A549-specific, respectively, suggesting that GR induces the expression of different sets of genes in a cell type-specific manner. RT-qPCR confirmed that the epithelial sodium channel (ENACα) gene is an ARPE-19 cell-specific GR target gene, whereas the FK506 binding protein 5 (FKBP5) gene was A549 cell-specific. There was a significant decrease in ENACα expression in FOXA1-deficient ARPE-19 cells, suggesting that FOXA1 might function as a pioneer factor enabling the selective expression of ENACα in ARPE-19 cells but not in A549 cells. CONCLUSION: These findings indicate that ENACα expression in ARPE-19 cells is regulated by FOXA1 and provide insights into the molecular mechanisms of cell type-specific expression of GR-regulated genes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.relation.isPartOf | International journal of immunopathology and pharmacology | - |
dc.title | Cell-specific expression of ENACα gene by FOXA1 in the glucocorticoid receptor pathway | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000565494800001 | - |
dc.identifier.doi | 10.1177/2058738420946192 | - |
dc.identifier.bibliographicCitation | International journal of immunopathology and pharmacology, v.34 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85089926429 | - |
dc.citation.title | International journal of immunopathology and pharmacology | - |
dc.citation.volume | 34 | - |
dc.contributor.affiliatedAuthor | Chung, Y.S. | - |
dc.contributor.affiliatedAuthor | Jeong, K.W. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | ENACα | - |
dc.subject.keywordAuthor | FOXA1 | - |
dc.subject.keywordAuthor | glucocorticoid receptor | - |
dc.subject.keywordAuthor | pioneer factor | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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