All-trans retinoic acid increases NF-κB activity in PMA-stimulated THP-1 cells upon unmethylated CpG challenge by enhancing cell surface TLR9 expression
DC Field | Value | Language |
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dc.contributor.author | Trinh, T.-A. | - |
dc.contributor.author | Hoang, T.X. | - |
dc.contributor.author | Kim, J.Y. | - |
dc.date.available | 2020-09-16T02:35:27Z | - |
dc.date.created | 2020-07-15 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 0300-8177 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78230 | - |
dc.description.abstract | An active metabolite of vitamin A, all-trans retinoic acid (ATRA), is known to exert immunomodulatory functions. This study investigates the possible immune potentiating effect of ATRA on NF-κB activity in human monocytic THP-1 cells after exposure to unmethylated CpG DNA ODN2006. We observed that challenge with ODN2006 significantly enhanced the NF-κB activity of PMA-differentiated THP-1 cells. ATRA synergistically enhanced NF-κB activity of cells, in a concentration- and time-dependent manner. The enhanced NF-κB activity of PMA-differentiated THP-1 cells after ODN2006 challenge was dependent on the RAR/RXR pathway. To determine the mechanism involved in increasing in the NF-κB activity of stimulated THP-1 cells, we examined the effects of PMA and ATRA on the expression of TLR9 (a receptor of ODN2006) in THP-1 cells. PMA treatment significantly enhanced both the intracellular and cell surface expression of TLR9, while ATRA alone showed no effect. However, ATRA synergistically enhanced the cell surface TLR9 expression of PMA-differentiated cells. To determine whether the ATRA-enhanced NF-κB activity is due to the enhanced cell surface TLR9 expression, we examined NF-κB activity after treatment with anti-TLR9 blocking antibody. Results revealed that the anti-TLR9 antibody treatment almost completely reverses the ATRA-enhanced NF-κB activity, suggesting that ATRA enhances NF-κB activity through upregulation of the cell surface TLR9 expression in PMA-differentiated and unmethylated CpG challenged THP-1 cells. © 2020, Springer Science+Business Media, LLC, part of Springer Nature. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Springer | - |
dc.relation.isPartOf | Molecular and Cellular Biochemistry | - |
dc.title | All-trans retinoic acid increases NF-κB activity in PMA-stimulated THP-1 cells upon unmethylated CpG challenge by enhancing cell surface TLR9 expression | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000546211300004 | - |
dc.identifier.doi | 10.1007/s11010-020-03817-4 | - |
dc.identifier.bibliographicCitation | Molecular and Cellular Biochemistry, v.173, no.1, pp.167 - 177 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85087566297 | - |
dc.citation.endPage | 177 | - |
dc.citation.startPage | 167 | - |
dc.citation.title | Molecular and Cellular Biochemistry | - |
dc.citation.volume | 173 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Trinh, T.-A. | - |
dc.contributor.affiliatedAuthor | Hoang, T.X. | - |
dc.contributor.affiliatedAuthor | Kim, J.Y. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | All-trans retinoic acid | - |
dc.subject.keywordAuthor | ODN2006 | - |
dc.subject.keywordAuthor | Phorbol-12-myristate-13-acetate | - |
dc.subject.keywordAuthor | THP-1 | - |
dc.subject.keywordAuthor | Toll-like receptor 9 | - |
dc.subject.keywordAuthor | Unmethylated CpG | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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