저온 노출된 혈관 세포 내 RhoA 단백질 활성화에 대한 십전대보탕의 억제 효과
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이강욱 | - |
dc.contributor.author | 김명선 | - |
dc.contributor.author | 김윤경 | - |
dc.contributor.author | 황현하 | - |
dc.contributor.author | 고호연 | - |
dc.contributor.author | 선승호 | - |
dc.contributor.author | 최유경 | - |
dc.contributor.author | 양승보 | - |
dc.contributor.author | 송윤경 | - |
dc.contributor.author | 전찬용 | - |
dc.contributor.author | 고성규 | - |
dc.date.available | 2020-10-20T00:59:20Z | - |
dc.date.created | 2020-10-20 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1226-9174 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78320 | - |
dc.description.abstract | Objective: To investigate the vasodilatory effect of Sipjeondaebo-tang by inhibiting RhoA activity in vascular cells during cold exposure. Methods: Human vascular endothelial cells and pericytes were pretreated with Sipjeondaebo-tang for 30 min, followed by incubation at 37 ℃ (control) or 25 ℃ (cold exposure) for 30 min. Activation of endothelin-1-mediated RhoA in pericytes was assessed by pretreating the cells with Sipjeondaebo-tang for 30 min, followed by incubation with endothelin-1 at 37 ℃ for 30 min. Western blotting was performed to measure the expression of active RhoA. Endothelin-1 and nitric oxide release from endothelial cells was examined with enzyme-linked immunosorbent assay kits. The formation of stress fibers and focal adhesion complexes was analyzed by immunocytochemistry. Results: Cold treatment activated RhoA in both pericytes and vascular endothelial cells, whereas Sipjeondaebo-tang treatment inhibited this activation. Sipjeondaebo-tang treatment also reversed the cold-mediated production of endothelin-1 and nitric oxide. Cold exposure promoted the formation of stress fibers and focal adhesion complexes by increasing the expression of phospho-focal adhesion complex kinase, whereas Sipjeondaebo-tang treatment suppressed this response. Conclusions: These findings suggested that Sipjeondaebo-tang inhibits cold-induced RhoA activation and its related pathway components, including endothelin-1 and nitric oxide, in vascular cells. Therefore, Sipjeondaebo-tang could be beneficial for the treatment of Raynaud’s phenomenon. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한한방내과학회 | - |
dc.relation.isPartOf | 대한한방내과학회지 | - |
dc.title | 저온 노출된 혈관 세포 내 RhoA 단백질 활성화에 대한 십전대보탕의 억제 효과 | - |
dc.title.alternative | Mitigative Effect of Sipjeondaebo-tang on RhoA Activation in Cold-Exposed Vascular Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.doi | 10.22246/jikm.2020.41.4.553 | - |
dc.identifier.bibliographicCitation | 대한한방내과학회지, v.41, no.4, pp.553 - 562 | - |
dc.identifier.kciid | ART002637091 | - |
dc.citation.endPage | 562 | - |
dc.citation.startPage | 553 | - |
dc.citation.title | 대한한방내과학회지 | - |
dc.citation.volume | 41 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | 최유경 | - |
dc.contributor.affiliatedAuthor | 양승보 | - |
dc.contributor.affiliatedAuthor | 송윤경 | - |
dc.contributor.affiliatedAuthor | 전찬용 | - |
dc.subject.keywordAuthor | Raynaud’s phenomenon | - |
dc.subject.keywordAuthor | cold | - |
dc.subject.keywordAuthor | rhoA | - |
dc.subject.keywordAuthor | endothelin-1 | - |
dc.subject.keywordAuthor | nitric oxide | - |
dc.subject.keywordAuthor | Sipjeondaebo-tang | - |
dc.description.journalRegisteredClass | kci | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.